B6.129 1 gt rosa 26sortm1 eyfp cos j

Manufactured by Jackson ImmunoResearch

B6.129×1-Gt(Rosa)26Sortm1(EYFP)Cos/J is a transgenic mouse strain that expresses the enhanced yellow fluorescent protein (EYFP) under the control of the Rosa26 promoter. This mouse line can be used for lineage tracing and other applications that require fluorescent protein expression.

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B6 cg gt rosa 26sortm14 cag tdtomato hze j, by Jackson ImmunoResearch (1 mentions) B6.129p2 cg rorctm2litt j, by Jackson ImmunoResearch (1 mentions) B6.129 gt rosa 26sortm1 cre ert2 tyj j, by Jackson ImmunoResearch (1 mentions)

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Gt(ROSA)26Sortm1(EYFP)Cos/J (10 citations)

4 protocols using b6.129 1 gt rosa 26sortm1 eyfp cos j

Genetic Labeling of Astrocytes

Cited 2 times

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GLAST-CreER^T2 transgenic mice^{12 (link)} were crossed to the Rosa26-enhanced yellow fluorescent protein (EYFP)^{47 (link)} or Rosa26-tdTomato^{48 (link)} Cre-reporter lines (obtained from the Jackson Laboratory, B6.129×1-Gt(Rosa)26Sor^tm1(EYFP)Cos/J, JAX stock: 006148; B6.Cg-Gt(Rosa)26Sor^{tm14(CAG-tdTomato)Hze}/J, JAX stock: 007914) to generate GLAST-CreER^T2;R26R-EYFP or GLAST-CreER^T2;R26R-tdTomato mice in which CreER^T2 is hemizygous and Rosa26-EYFP or Rosa26-tdTomato is either heterozygous or homozygous. For a set of experiments, GLAST-CreER^T2;R26R-EYFP were further crossed to Rasless mice^{27 (link)} to generate GLAST-CreER^T2;Rasless;R26R-EYFP mice, hereafter referred to as GLAST-Rasless-EYFP mice. All Rasless mice used were homozygous for H-Ras and N-Ras null alleles and homozygous for floxed K-Ras alleles.
All animals were in a C57BL/6J genetic background and ≥8 weeks old at the onset of experiments. Animals were housed in group in a pathogen-free facility with controlled humidity (50 ± 15%) and temperature (22 ± 2 °C), with 12:12-h light:dark cycles and free access to food and water. All experimental procedures were performed in accordance to the Swedish and European Union guidelines and approved by the institutional ethical committees (Stockholms Norra Djurförsöksetiska Nämnd or Malmö/Lunds Djurförsöksetiska Nämnd).

Dias D.O., Kalkitsas J., Kelahmetoglu Y., Estrada C.P., Tatarishvili J., Holl D., Jansson L., Banitalebi S., Amiry-Moghaddam M., Ernst A., Huttner H.B., Kokaia Z., Lindvall O., Brundin L., Frisén J, & Göritz C. (2021). Pericyte-derived fibrotic scarring is conserved across diverse central nervous system lesions. Nature Communications, 12, 5501.

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Fate Mapping of T Helper Subsets

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C57BL/6 (B6), B6.SJL-Ptprc^a Pepc^b/BoyJ (Cd45.1 mice), B6.129P2(Cg)-Rorc^tm2Litt/J (Rorγ^−/−), STOCK Il-17a^{tm1.1(icre)Stck}/J, B6.129 × 1-Gt(ROSA)26Sor^tm1(EYFP)cos/J, B6.129(Cg)-Foxp3^{tm3(DTR/GFP)Ayr}/J (Foxp3^GFP) and C57BL/6-Foxp3^tm1Flv/J (Foxp3^mRFP) mice were obtained from The Jackson Laboratory. IL-17 fate reporter mice (Il17a^CreRosa26^eYFP) were created in our laboratory by breeding STOCK IL17a^{tm1.1(icre)Stck}/J and B6.129 × 1-Gt(ROSA)26Sor^tm1(EYFP)cos/J. Foxp3 fate reporter mice (Foxp3^YFP/CreRosa26^tdTomato) were created in our laboratory by breeding B6.129(Cg)-Foxp3^{tm4(YFP/cre)Ayr/J} and B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J. IL-17 fate reporter mice were bred with Foxp3^mRFP mice to create Th17^eYFP-Foxp3^mRFP fate reporter (Il17a^CreRosa26^eYFP xFoxp3^tm1Flv) mice. All animal experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee (15045926). Six- to eight-week-old female mice were used in the experiments. In all experiments tumour-bearing mice were randomly allocated to experimental groups. In experiments where in vivo immunological assays were performed, the variation within groups allowed the detection of differences with 4–5 mice per group.

Downs-Canner S., Berkey S., Delgoffe G.M., Edwards R.P., Curiel T., Odunsi K., Bartlett D.L, & Obermajer N. (2017). Suppressive IL-17A+Foxp3+ and ex-Th17 IL-17AnegFoxp3+ Treg cells are a source of tumour-associated Treg cells. Nature Communications, 8, 14649.

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Transgenic Mice for Cardiac Studies

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The BAC transgene Tbx5^CreERT2 was constructed from the BAC clone RP23-267B15^{70 (link)} by replacement of exon 2 of Tbx5 with a CreERT2 cassette at the first methionine of the open reading frame in EL250 cells^{23 (link),71 (link)}. The BAC-Tbx5^CreERT2 transgenes were crossed with Rosa26R^eYFP/eYFP transgenic mice (B6.129×1-Gt(ROSA)26Sortm1(EYFP)Cos/J) from Jackson laboratories stock# 006148, in order to produce Tbx5^CreERT2/Rosa26R^eYFP/eYFP mice employed in this study. The Tbx5^CreERT2/+/Rosa26R^eYFP/+/Rosa26R^iDTR/+ was created using the tamoxifen-induced Rosa26R^iDTR/iDTR transgene^{72 (link)} (Jackson laboratories stock# 007900) provided by the Klinakis lab (BRFAA).
All animal work has been approved by the BRFAA ethics committee and the Attica Veterinary Department (Animal Licence; 60876/23-1-20). All animals used were 2–3 months of age upon the time of ischemia/reperfusion (I/R) or isoproterenol administration experiments following relevant inclusion/exclusion guideline criteria^{73 (link)}.

Siatra P., Vatsellas G., Chatzianastasiou A., Balafas E., Manolakou T., Papapetropoulos A., Agapaki A., Mouchtouri E.T., Ruchaya P.J., Korovesi A.G., Mavroidis M., Thanos D., Beis D, & Kokkinopoulos I. (2023). Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart. NPJ Regenerative Medicine, 8, 13.

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Generating Oct1-Deficient Mouse ESCs

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All mice were C57BL/6J background. Oct1 germline-deficient ESCs were generated by intercrossing heterozygous Pou2f1^-/+ mice (Wang et al., 2004 (link)) to generate a 1:2:1 ratio of Pou2f1^-/-: Pou2f1^-/+: Pou2f1^+/+ embryonic offspring. ESCs were derived from preimplantation blastocysts and genotyped. Heterozygous ESCs were not studied further. Littermate WT ESCs lines constituted the controls for these experiments. Oct1 inducible-conditional ESCs were generated by first separately crossing mice with the Pou2f1 conditional (floxed) allele (Shakya et al., 2015b (link)) to the YFP reporter B6.129 × 1-Gt(ROSA)26Sor^tm1(EYFP)Cos^/J (Jackson labs #006148) and inducible cre transgenic line B6.129-Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}^/J (Jackson labs #008463). Resulting Pou2f1^fl/fl animals were intercrossed to generate embryonic Pou2f1^fl/fl offspring in which LSL-YFP was expressed from one Rosa26 allele and Cre-ERT2 was expressed from the other. Parent ESCs were derived from these preimplantation blastocysts. The parent lines constituted the controls for derived 4-OHT-treated, Pou2f1^Δ/Δ:YFP⁺ lines. Cell lines were routinely authenticated by genotyping. Mycoplasma testing was conducted regularly in-house using a previously published method (Molla Kazemiha et al., 2009 (link)). Cells were negative throughout the study.

Shen Z., Kang J., Shakya A., Tabaka M., Jarboe E.A., Regev A, & Tantin D. (2017). Enforcement of developmental lineage specificity by transcription factor Oct1. eLife, 6, e20937.

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