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The fungus was cultivated in PDA at 28–30 °C for seven days, and all the colonies of a Petri dish (60 × 15 mm) were transferred to a 2 L Erlenmeyer containing 500 mL of PD. The culture was kept for 15 days at 28 °C and 150 rpm. The biomass was removed through filtration in cheesecloth, and the supernatant was submitted to solid phase extraction (SPE) in a C18 cartridge (Speed SPE Cartridges—Octadecyl C18/18%) using methanol at 20 and 100% as eluent, resulting in fractions SPE20 and SPE100, respectively. The fraction SPE100 was dried with rotaevaporation (Bucchi© R-210, Flawil, Switzerland) and purified through high performance liquid chromatography (HPLC) using a C18 column (Ace^®, 250 × 4.6 mm) with the isocratic method in two steps around 40–56% of mobile phase B, according to the impurities present (mobile phase A: trifluoroacetic acid (TFA) 0.1%; B: water/methanol/TFA 90:9.9:0.1%), leading to the obtaining of pure monocerin. The chemical structure of monocerin was determined by proton and carbon nuclear magnetic resonance (¹H-NMR, ¹³C-NMR) (Agilent 500/54 premium shielded) and EIMS, as previously described [6 (link),7 (link),15 ], and the purity was confirmed by gas chromatography-mass spectrometry (Agilent GC/MS, 7890/5975C).