Moflo astrios cell sorter

Manufactured by Agilent Technologies

The MoFlo Astrios cell sorter is a high-performance flow cytometry instrument designed for cell sorting applications. It is capable of accurately and efficiently separating individual cells from a heterogeneous population based on their physical and fluorescent characteristics.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Diva program, by BD (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Agilent Technologies (1 mentions)

Close spelling variants of this product

MoFlo cell sorter MoFlo sorter

2 protocols using moflo astrios cell sorter

Flow Cytometric Analysis of Lamina Propria Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell suspensions from the lamina propria were prepared as described previously^{36 (link)}. All cells were first pre-incubated with anti-mouse CD16/CD32 for blockade of Fc γ receptors, then were washed and incubated for 40 min with the appropriate monoclonal antibody conjugates in a total volume of 200 μl PBS containing 2 mM EDTA and 2% (vol/vol) bovine serum. DAPI (Invitrogen) was used to distinguish live cells from dead cells during cell analysis and sorting. For detection of intracellular Foxp3, a Foxp3 Staining Buffer Set (eBioscience) was used for fixation and permeabilization of the cells. Stained cells were analyzed on a LSRII machine using Diva program (BD Bioscience) or purified with a MoFlo Astrios cell sorter (DakoCytomation). Cells were >98% pure after sorting. Data were analyzed with FlowJo software (TreeStar).

He Z., Chen L., Souto F.O., Canasto-Chibuque C., Bongers G., Deshpande M., Harpaz N., Ko H.M., Kelley K., Furtado G.C, & Lira S.A. (2017). Epithelial-derived IL-33 promotes intestinal tumorigenesis in ApcMin/+ mice. Scientific Reports, 7, 5520.

+ Open protocol

+ Expand

Isolation of Intestinal Epithelial and Lamina Propria Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell pellets were first pre-incubated with anti-mouse CD16/CD32 for blockade of Fc γ receptors, then were washed and incubated for 30 min with fluorescent conjugated antibodies against CD45 and EpCAM in a total volume of 500 μl PBS containing 2 mM EDTA and 2% (vol/vol) fetal bovine serum. DAPI (Invitrogen) was used to distinguish live cells from dead cells during cell sorting. Stained IECs (DAPI⁻CD45⁻Epcam⁺) and LPL (DAPI⁻CD45⁺) were purified with a MoFlo Astrios cell sorter (DakoCytomation). Cells were >95% pure after sorting.

He Z., Chen L., Furtado G.C, & Lira S.A. (2018). Interleukin 33 regulates gene expression in intestinal epithelial cells independently of its nuclear localization. Cytokine, 111, 146-153.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!