Caspase-8 activity was determined by Caspase-8/FLICE fluorometric assay kit (Biovision, U.S.A.) as previously described27 (link). The result represents fold activity of caspases-8 with respect to control cells. Next, caspase-3 activity was determined by EnzChek Caspase-3 Assay Kit (Invitrogen, Life Technologies, USA) as previously described63 (link). The results represented as fold increase in the activity of caspases-8 and caspase-3 with respect to control.
Caspase 8 flice fluorometric assay kit
Manufactured by Abcam
Sourced in United States
The Caspase-8/FLICE fluorometric assay kit is a laboratory tool designed to detect and quantify the activity of caspase-8, a key enzyme involved in the cellular apoptosis (programmed cell death) pathway. The kit utilizes a fluorogenic substrate that emits a fluorescent signal upon cleavage by active caspase-8, allowing for the measurement of caspase-8 activity in cell lysates or purified samples.
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Lab products found in correlation
2 protocols using caspase 8 flice fluorometric assay kit
1
Caspase-8 and Caspase-3 Activity Assay
2
Caspase-8 and Caspase-3 Activity Assays
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The fold induction of caspase-8 activity was determined by a Caspase-8/FLICE fluorometric assay kit (Biovision) as previously described [19 (link)]. Next, the fold induction caspase-3 activity was determined by an EnzChek Caspase-3 Assay Kit (Life Technologies) as previously described [70 (link)]. The result represents the fold activity of caspase-3 with respect to control cells.
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