Silicon wafers with desired patterns were made using SU-8 photoresist for soft lithography34 . To form stamps, PDMS (Sylgard 184, Dow Corning) at 1:10 mixture ratio (curing agent: silicone elastomer) was molded onto silanized wafers and cured at 80 °C for 2 hr. A mixture of 50 μg/ml pure fibronectin (Roche) and 25 μg/ml conjugated fibronectin (Cy5.5 or Atto dye, GE Healthcare and Sigma) was incubated on the stamps for 1 hr and dried before stamping. Patterns were stamped onto a UV-activated, PDMS spin-coated petri dish and the unstamped area was passivated by 1% Pluronics (F127, Sigma) for 1 hr. Samples were rinsed with PBS before cell seeding.
Atto dye
Manufactured by GE Healthcare
Atto dye is a fluorescent labeling agent used in various biomedical applications. It is designed to allow the detection and visualization of biological molecules, such as proteins, nucleic acids, and other biomolecules, in research and diagnostic settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using atto dye
1
Soft Lithography for Fibronectin Patterning
2
Soft Lithography for Fibronectin Patterning
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