Uv 3101pc uv vis nir

Chl_a+b was calculated using the approach described in [53 (link)]. Using a cork borer, four discs were extracted from the middle of a banana leaf and placed in a plastic vial containing 20 mL of 80% acetone (readily covered with aluminum foil). The samples were kept in the dark for 7 days to extract all of the pigments. The absorbance values of the solution from each sample were read at 647 nm and 664 nm using a spectrophotometer (UV-3101PC UV-VIS-NIR, Shimadzu, Kyoto, Japan) to determine Chl_a, Chl_b and Chl_{a = b} contents, which were calculated as follows:



where Chl_a, Chl_b and Chl_a+b denote chlorophyll a, chlorophyll b and total chlorophyll (a + b), respectively. A₆₄₇ and A₆₆₄ represent the absorbance of the solution at 647 and 664 nm, respectively; 13.19, 2.57, 22.1 and 5.26 are the absorption coefficients; 3.5 is the total volume used in the analysis, obtained from the original solution (mL); and 4 is the total disc area (cm²).

Absorption spectra were obtained in a Shimadzu UV-3101PC UV–Vis-NIR (Shimadzu Corporation, Kyoto, Japan) spectrophotometer. Fluorescence spectra were recorded in a Fluorolog 3 spectrofluorimeter (HORIBA Jobin Yvon IBH Ltd., Glasgow, UK), all spectra being corrected for the instrumental response. The steady-state fluorescence anisotropy, r, was measured in the latter equipment, using Glan–Thompson polarizers.
FRET assays were carried out as previously reported [22 (link),24 (link),41 ], allowing confirming the formation of the double lipid layer in SMLs and the interactions with model membranes. The detailed procedure is described in the Supplementary Material. The fluorescence quantum yield of the dye NBD (donor) in magnetoliposomes (containing the different magnetic nanoparticles) was determined by the standard method [60 ,61 (link)], using the labeled lipid NBD-C₁₂-HPC in lipid membranes as reference, with Φ_r = 0.32 at 25 °C [62 ].