Anti cd11b pe clone m1 70

Manufactured by BioLegend

Sourced in United States

Anti CD11b-PE (Clone M1/70) is a laboratory reagent used for the identification and detection of CD11b, a cell surface marker expressed on various immune cells such as monocytes, macrophages, and granulocytes. It is conjugated with the fluorescent dye phycoerythrin (PE) for flow cytometric analysis.

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Lab products found in correlation

Anti cd34 pe, by BioLegend (1 mentions) Autostainer link 48, by Agilent Technologies (1 mentions) Anti cd14 pe clone m5e2, by BioLegend (1 mentions) Propridium iodide, by Merck Group (1 mentions) Collagenase 4, by Thermo Fisher Scientific (1 mentions) Cyan flow cytometer, by Agilent Technologies (1 mentions) Streptavidin fitc, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD11b (M1/70, (86 citations) CD11b-PE (81 citations) CD11b (clone M1/70, (72 citations) Anti-CD11b-PE (52 citations) M1/70 (46 citations) Anti-CD11b (clone M1/70, (45 citations) Anti-CD11b (M1/70, (42 citations) Clone M1/70 (26 citations) CD11b PE (M1/70, (7 citations) CD11b PE (clone M1/70, (6 citations)

2 protocols using anti cd11b pe clone m1 70

Enhanced CTC Identification Protocol

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The same cell staining reagents and the same reaction time were used for the CTC-FIND method and the RareCyte method. Specifically, cell staining was performed using a proprietary custom reagent kit developed by RareCyte. This included the following: 4′,6-diamidino-2-phenylindole (DAPI) to stain the cell nucleus) and antibodies specific for the epithelial marker CK, the epithelial marker EpCAM, and the cell surface antigen CD45/CD66b (used for staining leukocytes). We modified the assay by adding additional counter-stain reagent with the following phycoerythrin (PE)-conjugated antibodies: anti-CD14-PE (Clone M5E2, 1:200, BioLegend, San Diego, CA, USA), anti-CD34-PE (Clone 581, 1:200, BioLegend, San Diego, CA, USA), and anti CD11b-PE (Clone M1/70, 1:200, BioLegend, San Diego, CA, USA). For the CTC-FIND method, all staining was done with the slit-filter isolation unit of CTC-FIND. For the RareCyte method, all staining was done with the AutoStainer Link 48 (Agilent Technologies, Santa Clara, CA, USA) and according to manufacturer’s protocol.

Takagi H., Dong L., Kuczler M.D., Lombardo K., Hirai M., Amend S.R, & Pienta K.J. (2020). Analysis of the Circulating Tumor Cell Capture Ability of a Slit Filter-Based Method in Comparison to a Selection-Free Method in Multiple Cancer Types. International Journal of Molecular Sciences, 21(23), 9031.

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Murine Corneal Immune Cell Profiling

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Whole corneal tissue was isolated from murine eyes, placed in ice cold PBS and cut into small fragments using a scalpel blade. Corneal fragments were then digested in 2mg/ml Collagenase IV (invitrogen)/PBS for 30-45 minutes at 37°C with gentle shaking. The resulting cell suspensions were then passed through a 70μm cell strainer into 1x HBSS/25mM HEPES/2% newborn bovine calf serum (staining medium). Cells were subsequently washed twice with staining medium and then stained according to standard flow cytometry protocols with the following monoclonal antibody conjugates: anti-CD45-Pacific Blue (clone 30-F11, Invitrogen), anti-CD11b-PE (clone M1/70, Biolegend), anti-Gr-1-alexa 647 (clone RB6-8C5, EPFL Protein Core Facility), anti-CD11c-APC-eFluor 780 (clone N418, eBiosciences), anti-B220-PECy7 (clone RA3-6B2, eBiosciences), anti-TCRB-APC-eFluor 780 (clone H57-597, eBiosciences), anti-CD4-PE (clone GK1.5, EPFL Protein Core Facility), anti-CD8-Alexa Fluor 647 (clone YTS169.4, EPFL Protein Core Facility), anti-F4/80-biotin (clone BM8, eBiosciences), Streptavidin-FITC (eBiosciences). Prior to analysis, Propridium iodide (Sigma) was added to cell suspensions at a final concentration of 5 μg/ml to enable dead cell exclusion. Data acquisition was performed using a Cyan flow cytometer (Dako) and analysed using FlowJo software (Tree Star).

Nowell C.S., Odermatt P.D., Azzolin L., Hohnel S., Wagner E.F., Fantner G.E., Lutolf M.P., Barrandon Y., Piccolo S, & Radtke F. (2015). Chronic inflammation imposes aberrant cell fate in regenerating epithelia via mechanotransduction. Nature cell biology, 18(2), 168-180.

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