Optical rotations ([α]25D) were measured on a PerkinElmer 241 polarimeter in a 100 × 2 mm cell (units 10−1 deg cm2 g−1). UV absorption spectra were obtained using a Varian Cary 50 Bio UV– visible spectrophotometer. NMR spectra were acquired on a Bruker Avance III spectrometer operating at 600 MHz for 1H and 150 MHz for 13C and equipped with a 3 mm cryogenically cooled probe. 1H and 13C spectra were referenced to the residual deuterated solvent peaks. HRESIMS data were acquired on an Agilent 6520 Accurate Mass Q-TOF instrument with internal reference masses calibrated at 121.05087 and 922.00979, both within 5 ppm. Diol SPE fractionation of the extract was performed on DIO Spe-ed SPE cartridges, and subsequent fractions were separated on a RP-C18 column attached to a model UA-6 UV detector and Foxy 200 fraction collector (Teledyne Isco). Final purifications were performed using a Varian ProStar 218 solvent delivery module HPLC equipped with a Varian ProStar 325 UV– vis detector, operating under Star 6.41 chromatography workstation software. All solvents and chemicals used were of analytical grade.
Prostar 325 uv vis detector
Manufactured by Agilent Technologies
Sourced in United States
The ProStar 325 UV–vis detector is a laboratory instrument used for the detection and quantification of compounds that absorb ultraviolet or visible light. It measures the absorbance of a sample at specific wavelengths, providing data for analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Galaxy software, by Agilent Technologies (3 mentions)
Foxy 200 fraction collector, by Teledyne (1 mentions)
6520 accurate mass q tof, by Agilent Technologies (1 mentions)
Cary 50 bio, by Agilent Technologies (1 mentions)
Avance 3, by Bruker (1 mentions)
J 710, by Jasco (1 mentions)
Prostar hplc separation system, by Agilent Technologies (1 mentions)
Gemini column, by Phenomenex (1 mentions)
Varian prostar hplc system, by Agilent Technologies (1 mentions)
Prostar 210 pump, by Agilent Technologies (1 mentions)
Prostar 410 autosampler, by Agilent Technologies (1 mentions)
6 protocols using prostar 325 uv vis detector
1
Spectroscopic Analysis of Natural Products
2
High-Resolution Mass Spectrometry and NMR Characterization
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High-resolution ESI-MS and HR-ESI-MS-HPLC experiments were performed on a Thermo LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific Spa, Rodano, Italy) coupled to an Agilent model 1100 LC system (Agilent Technology Cernusco sul Naviglio, Italia). The spectra were recorded by infusion into the ESI source using MeOH as the solvent. CD spectra were recorded using a Jasco J-710 (Easton, MD) spectrophotometer using a 1 mm cell. NMR experiments were performed on Varian Unity Inova spectrometers (Agilent Technology Cernusco sul Naviglio, Italia) at 700 MHz in CD3OD; chemical shifts were referenced to the residual solvent signal (CD3OD: δH 3.31, δC 49.00). All 13C chemical shift were assigned using the 2D spectra, therefore, mono-dimensional 13C NMR spectra were not recorded (see Tables S1 and S2 ). For an accurate measurement of the coupling constants, the one-dimensional 1H NMR spectra were transformed at 64 K points (digital resolution: 0.09 Hz). Through-space 1H connectivities were evidenced using a ROESY experiment with a mixing time of 450 ms. The HSQC spectra were optimized for 1JCH = 142 Hz and the HMBC experiments for 2,3JCH = 8.3 Hz. High-performance liquid chromatography (HPLC) separations were achieved on a Varian Prostar 210 apparatus equipped with a Varian Prostar 325 UV-Vis detector.
3
HPLC Analysis of Plant Phytochemicals
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High-performance liquid chromatography (HPLC) was performed on a Varian ProStar HPLC separation system coupled with a ProStar 210 solvent delivery module, a ProStar 325 UV-Vis detector (Varian Inc., Walnut Creek, CA, USA), and a Gemini column (250 mm × 4.6 mm) packed with octadecyl group bonded type silica gel (C18)—pore size 110 Å, particle diameter 5 μm (Phenomenex, Torrance, CA, USA). Prior to the analysis, the extracts were diluted 10-times and filtered through a 0.2-µm syringe nylon filter. The injection volume was 20 µL. The column thermostat was set at 25 °C and elution was carried out at a flow rate of 1 mL min−1. Mobile phase A contained 0.1% formic acid in H2O and mobile phase B contained 0.1% formic acid in acetonitrile. The elution gradient mode was as follows: 4% B (pre-run), 4–22% B (0–25 min), 22–25% B (25–40 min), 25–100% B (40–50 min), 100% B (50–55 min), 100–4% B (55–60 min), 4% B (60–65 min). The chromatograms were acquired at λ = 280 nm for ARB, mARB, PIC, COR, and PGG and at λ = 350 nm for HYP and MYR. Quantification of individual phytochemicals was performed using calibration curves for standards. The results were expressed in mg g−1 dry matter of plant material.
4
Biogenic Amines Quantification in Mushrooms
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Sample preparation and determination of biogenic amines (BA) in mushroom samples were performed according to the method of Ben-Gigirey et al. [30 (link)], with some modifications described by Bartkiene et al. [31 (link)]. Before the experiment, mushroom samples were homogenized using a blender. The following BA were analysed: tryptamine, phenylethylamine, cadaverine, putrescine, histamine, tyramine, spermine, and spermidine. The standard BA solutions were prepared by dissolving known amounts of each BA (including internal standard—1.7-diamino-heptane) in 20 mL of deionised water. Briefly, 5 g of sample were extracted with 10 mL of perchloric acid (0.4 mol/L) twice. The derivatization of sample extracts and standards was performed using a dansyl chloride solution in acetonitrile (10 mg/mL) as a reagent. A Varian ProStar HPLC system (Varian Corp., Palo Alto, CA, USA) equipped with a ProStar 325 UV/VIS Detector and Galaxy software (Agilent, Santa Clara, CA, USA) was used for analysis. A Discovery® HS C18 column (150 × 4.6 mm, 5 µm; SupelcoTM Analytical, Bellefonte, PA, USA) was used to separate BA. Ammonium acetate (0.1 mol/L) and acetonitrile were used as the mobile phases at a flow rate of 0.8 mL/min. The sample volume injected was 20 µL and the amines were monitored at 254 nm. The BA were identified based on their retention times in comparison to their corresponding standards.
5
HPLC Analysis of Biogenic Amines in Pineapple Samples
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Sample preparation and determination of BAs in Ps samples were performed according to the method of Ben‐Gigirey, Baptista de Sousa, Juan, Villa, and Barros‐Velazquez (1999 ). The chromatographic analyses were carried out using a Varian ProStar HPLC system (Varian Corp.) with two ProStar 210 pumps, a ProStar 410 autosampler, a ProStar 325 UV/VIS Detector, and Galaxy software (Agilent) for data processing. For the separation of amines, a Discovery ® HS C18 column (150 × 4.6 mm, 5 µm; SupelcoTM Analytical) was used. The eluents were ammonium acetate (A) and acetonitrile (B), and the elution program consisted of a gradient system with a 0.8 ml/min flow rate. The detection wavelength was set to 254 nm, the oven temperature was 40°C, and samples were injected in 20 μl aliquots. The target compounds were identified based on their retention times in comparison with their corresponding standards.
6
Biogenic Amine Determination in Bacillus Cereus
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The extraction and determination of BA in nonfermented and fermented BC samples followed the procedures developed by Ben-Gigirey et al. (1999) (link) with some modifications as described by Bartkiene et al. (2020b) . In 20 mL of deionized water, stock solutions for each BA (1 mg/mL), including the internal standard 1.7-diamino-heptane, were produced. In short, 10 mL of perchloric acid (0.4 mol/L) were used 2 times to extract 5 g of the BC.
Derivatization of sample extracts and standards was performed with a dansyl chloride solution in acetonitrile (10 mg/mL). Varian ProStar HPLC system (Varian Corp., Palo Alto, CA) equipped with ProStar 325 UV/VIS Detector and Galaxy software (Agilent, Santa Clara, CA) was employed. A Discovery HS C18 column 150 mm length × 4.6 mm-ϕ, 5 μm-ϕ particle size; SupelcoTM Analytical, Bellefonte, Pennsylvania) was used for separation. Ammonium acetate 0.1 mol/L (solvent A) and acetonitrile (solvent B) were used as mobile phases at constant flow rate of 0.8 mL/min. The detection limit of BA was 0.1 mg/kg. The BA were identified based on their retention times in comparison to their corresponding standards.
Derivatization of sample extracts and standards was performed with a dansyl chloride solution in acetonitrile (10 mg/mL). Varian ProStar HPLC system (Varian Corp., Palo Alto, CA) equipped with ProStar 325 UV/VIS Detector and Galaxy software (Agilent, Santa Clara, CA) was employed. A Discovery HS C18 column 150 mm length × 4.6 mm-ϕ, 5 μm-ϕ particle size; SupelcoTM Analytical, Bellefonte, Pennsylvania) was used for separation. Ammonium acetate 0.1 mol/L (solvent A) and acetonitrile (solvent B) were used as mobile phases at constant flow rate of 0.8 mL/min. The detection limit of BA was 0.1 mg/kg. The BA were identified based on their retention times in comparison to their corresponding standards.
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