The largest database of trusted experimental protocols

20 ci mmol

Manufactured by PerkinElmer
Sourced in United States

The 20 Ci/mmol is a laboratory equipment product that provides a specific radioactivity measurement. This product offers a core function of quantifying the amount of radioactive material present in a sample. The numerical value indicates the relationship between the radioactivity and the amount of the material.

Automatically generated - may contain errors

2 protocols using 20 ci mmol

1

Metabolic Regulation of Cell Cycle and Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gelatin, sodium dodecyl sulfate (SDS), dithiothreitol, NaCl, EDTA, heparin, trichloroacetic acid, trypan blue, propidium iodide, RNase, cesium chloride, M199 medium, dimethyl-α-ketoglutarate, aspartate, hydrogen peroxide, glutamine, dialyzed fetal bovine serum, trypsin, 6-diazo-5-oxo-L-norleucine (DON), mercaptoethanol, streptomycin, penicillin, bis-2-(5-phenylacetomido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES), glutamate, ammonium chloride, and diethylamine NONOate (DEA-NO), K2HPO4, ATP, NAD, hydrazine, bovine liver glutamate dehydrogenase, and asparagine were from Sigma-Aldrich (St. Louis, MO). Endothelial cell growth factor was from Becton Dickinson Biosciences (Bedford, MA). Rainbow molecular weight markers were from GE Healthcare (Piscataway, NJ). Lipofectamine and 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) were from Life Technologies Corporation (Carlsbad, CA). CB-839 and NG-nitro-L-arginine methyl ester (L-NAME), were from Selleckchem (Houston, TX). Antibodies against GLS1, cyclin D1, cyclin E, cyclin A, p21, p27, p53, and β-actin were from Santa Cruz Biotechnology (Santa Cruz, CA), an antibody against phospho-retinoblastoma protein was from Cell Signaling Technologies (Beverley, MA), and an antibody against HO-1 was from Assay Designs (Ann Arbor, MI). [3H]Thymidine (20 Ci/mmol) was from Perkin Elmer (Boston, MA).
+ Open protocol
+ Expand
2

Cell Culture Materials and Methods

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco’s modified Eagle medium (DMEM), fetal calf serum (FCS), Hank’s balanced salt solution (HBSS) and other materials used for cell cultures were purchased from Gibco BRL (Carlsbad, CA, USA). 3,5-Dihydroxybenzoic acid (DHBA), Methylthiazolyldiphenyl-tetrazolium bromide (MTT), DL-Fluorocitric acid barium salt, cytochalasin B, GSH standard stock solution, 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), o-phthaldialdehyde, γ-glutamylhydroxamate, o-Phenylenediamine, S100B protein, anti-S100B antibody (SH-B1) and anti-GPR81-S296 antibody were purchased from Sigma Aldrich (St Louis, MO, USA). Peroxidase secondary antibody was purchased from GE (Little Chalfont, United Kingdom). Anti-β-actin conjugated with HRP was purchased from Protein Tech (Chicago, IL, USA). Polyclonal anti-S100B (clone SH-B) was purchased from Dako (São Paulo, Brazil). Deoxy-[3-3H] glucose (20 Ci/mmol) and l-[3,4-3H] glutamate (0.33 μCi/mL) were purchased from PerkinElmer (Boston, MA, USA). Non-described reagents were purchased from local commercial suppliers (Sulquímica or Labsul, Porto Alegre, Brazil).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!