Ab172730

Manufactured by Thermo Fisher Scientific

The Ab172730 is a laboratory equipment designed for specific applications. It serves as a tool for researchers and scientists in various fields. The core function of this product is to perform targeted tasks within the laboratory environment.

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Lab products found in correlation

Dynabeads protein g, by Thermo Fisher Scientific (2 mentions) Mab227, by R&D Systems (1 mentions) Protease inhibitors cocktail, by Selleck Chemicals (1 mentions) Ab42364, by Proteintech (1 mentions)

2 protocols using ab172730

Immunoprecipitation and Immunoblot Analysis

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Cells with indicated treatments were lysed in IP buffer supplemented with protLytic protease and phosphatase inhibitor cocktail, EDTA‐Free, 100× in DMSO (NCM, P002). Then lysates were incubated with pre‐linked anti‐antibody (5 μg, Abcam, ab40797), or control rabbit IgG (5 μg, Abcam, ab172730) Dynabeads protein G (Life technologies, 10004D) for 2 h at RT, following immunoblot with SPON1 (1:1000, Abcam, ab14271), gp130 (1:1000, Proteintech, 67766‐1‐Ig), sIL‐6R (1:1000, MAB227, R&D).

Huo Y., Yang J., Zheng J., Xu D., Yang M., Tao L., Yao H., Fu X., Yang J., Liu D., Hua R., Zhang J., Sun Y., Hu L, & Liu W. (2022). Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL‐6 trans‐signalling. Cell Proliferation, 55(5), e13237.

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Immunoprecipitation of OPA1 and HSP90

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Cells with indicated treatments were lysed in IP buffer supplemented with protease inhibitors cocktail (B14001, bimake). Then lysates were incubated with pre-linked anti-OPA1 antibody (Abcam, ab42364), HSP90 (13171-1-AP, Proteintech), or control rabbit IgG (Abcam, ab172730) Dynabeads protein G (Life technologies, 10004D) for 2 h at room temperature, following immunoblot with indicated antibodies.

Hu L.P., Zhou K.X., Huo Y.M., Liu D.J., Li Q., Yang M.W., Huang P.Q., Xu C.J., Tian G.A., Yao L.L., Zhang X.L., Wang Y.H., Li J., Zhang Z.G., Jiang S.H., Xing X., Wang X., Qin W.T, & Yang Q. (2021). Single-cell RNA sequencing reveals that targeting HSP90 suppresses PDAC progression by restraining mitochondrial bioenergetics. Oncogenesis, 10(3), 22.

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