G1969 85000

Manufactured by Agilent Technologies

The G1969-85000 is a lab equipment product from Agilent Technologies. It is a core component designed to perform a specific function within a larger system or setup. The description and details of its intended use are not available at this time.

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Lab products found in correlation

5 protocols using g1969 85000

Quantitative LC-QTOF-MS Metabolomic Analysis

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LC-QTOF-MS experiments were performed using an Agilent 1200 SL LC system coupled online with an Agilent 6520 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA). Each prepared sample (4 μL for positive ESI ionization, 8 μL for negative ESI ionization) was injected onto an Agilent Zorbax 300 SB-C8 column (2.1 × 50 mm, 1.8-micron), which was heated to 50 °C. The flow rate was 0.4 mL/min. Mobile phase A was 5 mM ammonium acetate and 0.1% formic acid in water, and mobile phase B was 5% water in ACN containing 5 mM ammonium acetate and 0.1% formic acid. The mobile phase composition was kept isocratic at 35% B for 1 min, and was increased to 95% B in 19 min; after another 10 min at 95% B, the mobile phase composition was returned to 35% B. The ESI voltage was 3.8 kV. The mass accuracy of our LC-MS system is generally less than 5 ppm; the Q-TOF MS spectrometer was calibrated prior to each batch run, and a mass accuracy of less than 1 ppm was often achieved using the standard tuning mixture (G1969-85000, Agilent Technologies, Santa Clara, CA). The mass scan range is 100–1600, and the acquisition rate was 1.5 spectra/s. The absolute intensity threshold for MS data collection was set to 100, and the relative threshold was 0.001%.

Buas M.F., Gu H., Djukovic D., Zhu J., Drescher C.W., Urban N., Raftery D, & Li C.I. (2015). Identification of novel candidate plasma metabolite biomarkers for distinguishing serous ovarian carcinoma and benign serous ovarian tumors. Gynecologic oncology, 140(1), 138-144.

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Quantitative Analysis of Stilbenes by HPLC-QTOF

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The analytical system used was High Performance Liquid Chromatography Agilent 1100 series coupled to Agilent 6510 accurate-mass Quadrupole-Time of Flight (Q-TOF) Mass spectrometer with ESI interface in negative ionization mode (Agilent Technologies, California, USA). Data acquisition system software was Agilent MassHunter version B.02.00. A Zorbax SB-C18 column (3.1×150 mm, ∅ 3.5 μm), equipped with a 2.1x12.5 mm ∅ 5 μm Zorbax Eclipse plus C18 precolumn (Agilent Technologies), was used at 35°C and the injected volume was 2 μL. The elution gradient was carried out with binary solvent system consisting of 0.1% formic acid in H₂O (solvent A) and 0.1% formic acid in MeOH (solvent B) at a constant flow-rate of 0.35 mL.min^-1. The gradient elution program was as follows: 0−3.0 min, 5% B; 3.0−23.0 min, up to 100% B; held for 10.0 min, followed by 7 min of stabilization at 5% B.
The mass spectrometer operated by detection in scan mode with the following settings: drying gas 13.0 L.min^-1 at 325°C; nebulizer pressure 35 psi; capillary voltage -3500 V, fragmentor 150 V. Negative mass calibration was performed with standard mix G1969-85000 (Agilent Technologies).
Data processing was performed with Agilent MassHunter Qualitative and Quantitative software version B.07.00. Absolute stilbene contents were estimated from external calibration curves prepared with pure standards.

Stempien E., Goddard M.L., Wilhelm K., Tarnus C., Bertsch C, & Chong J. (2017). Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization. PLoS ONE, 12(12), e0188766.

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Synthetic Histone H3.1 Tail PTM Analysis

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A set of five histone H3.1 tail peptides (ARTKQTARKSTGGKAPRKQLATKAARK-SAPATGGVKKPHRYRPGTVALRE) was probed incorporating acetylation (AcK18 and AcK27) and trimethylation (TriMetK4, TriMetK9 and TriMetK27) PTMs in biologically relevant positions (MW 5381 Da).^{44 (link)} These histone tails are proteotypic for the endoproteinase Glu-C derived peptides from histone H3. The synthetic histone H3.1 tails were purchased from GenScript (Piscataway, NJ). H3.1 tail solutions were analyzed at a concentration of 5 μM in 50:50 water/methanol (H₂O/MeOH) solvent conditions. A low concentration tuning mix standard (G1969–85000, Agilent Technologies, Santa Clara, CA) was used for external mobility and mass calibration.

Dit Fouque K.J., Kaplan D., Voinov V.G., Holck F.H., Jensen O.N, & Fernandez-Lima F. (2021). Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Analytical chemistry, 93(27), 9575-9582.

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Phospholipid Profiling by LC-MS

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10 μL of the prepared sample containing phospholipid was injected into an Agilent 1200 HPLC system (Agilent). 50% (v/v) of water with 0.1% (v/v) formic acid and 50% (v/v) acetonitrile with 0.1% (v/v) formic acid was used as an isocratic mobile phase at a flow rate of 0.4 mL/min. The HPLC system was connected to an Agilent 6220 Accurate-Mass TOF (Agilent) equipped with an electrospray interface operating in positive ion mode. The optimized operation parameters were as follows: capillary voltage, 4000 V; nebulizer pressure, 30 psi; drying gas flow rate, 11 L/min; gas temperature, 673 K; skimmer voltage, 60 V; octapole rf, 250 V; fragment voltage (in-source CID fragmentation), 200 V. LC-MS accurate mass spectra were recorded across the range m/z 50–1000. The accurate-mass calibration was performed over a mass range of m/z 118.0863–2721.8950 using a calibration solution provided by the manufacturer (G1969-85000, Agilent, Santa Clara). The full-scan data were recorded with the Agilent Mass Hunter Data Acquisition software (version B.03.01) and processed with the Agilent Mass Hunter Qualitative Analysis software (version B.03.01).

Kim J.S., Park J., Kim M.S., Ha J.Y., Jang Y.W., Shin D.H, & Son J.H. (2017). The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin. Scientific Reports, 7, 6248.

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Lipidome Profiling of Cerebrospinal Fluid

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Global MS-based lipidomics was performed using an Agilent 1200 LC system coupled to an Agilent 6520 Q-TOF mass spectrometer. CSF samples (200 μL) were prepared by dissolving in 1000 μL methanol, which was vortexed, incubated at -20 °C and centrifuged at 18000 x g for 20 min. Then. 750 μL were collected, dried and then reconstituted in a 100 μL solution of 40% H2O 60% acetonitrile. Five μL of each prepared sample were analyzed by positive ESI ionization and 10 μL analyzed by negative ESI ionization. Samples were separated using a Waters XBridge BEH Amide column (15 cm x 2.1mm, 2.5 μm), which was heated to 40 °C. The mobile phase was 10 mM NH4HCO3 in 100% H2O (Solvent A) and 100 % acetonitrile (Solvent B) and its gradient was 95-10% B from 0 to 5 min, 10% B from 5-40 min., 10-100% B from 40-45 min., and 100% from 40-70 min. MS parameters were performed according to previously described methods (29) (link). The mass accuracy of our LC-MS system is generally better than 5 ppm, the Q-TOF/MS spectrometer was calibrated prior to each batch run, and a mass accuracy of <1 ppm was often achieved using the standard tuning mixture (G1969-85000, Agilent Technologies). The m/z scan range was 100-2000, and the acquisition rate was 1.0 spectra/s. MS data were processed using Agilent Mass
Hunter and Mass Profiler Professional.

Hwangbo N., Zhang X., Raftery D., Gu H., Hu S., Montine T.J., Quinn J.F., Chung K.A., Hiller A., Wang D., Fei Q., Bettcher L., Zabetian C.P., Peskind E.R., Li G., Promislow D., & Franks A. (2021). An aging clock using metabolomic CSF.

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