The analytical standard 5-CQA (CAS Number: 327-97-9) was provided by Carl Roth GmBH (purity: >97%, Karlsruhe, Germany). 3-CQA (CAS Number: 906-33-2, purity > 95%), 4-CQA (CAS Number: 905-99-7, >95%), caffeine (CAS Number: 58-05-2, purity > 99%), glacial acetic acid, and potassium phosphate monobasic were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-FQA (CAS Number: 1899-29-2, purity 98%) was supplied by Chem Faces (Hubei, China). 3,4-DiCQA (CAS Number: 14534-61-3, purity > 95.0%), 3,5-diCQA (CAS Number: 2450-53-5, purity > 95%), and 4,5-diCQA (CAS Number: 32451-88-0, purity > 95%) were obtained from Biopurity Phytochemicals (Sichuan, China). HPLC-grade acetonitrile and methanol were provided by J.T. Baker (Griesheim, Germany). A Barnstead Nanopure Diamond (Dubuque, IA, USA) was used to make refined deionized water.
3 cqa
Manufactured by Merck Group
Sourced in United States
3-CQA is a laboratory reagent used as a chemical standard in analytical and research applications. It is a white crystalline solid with the molecular formula C₁₅H₁₈O₆. 3-CQA is commonly used as a reference compound for quantitative and qualitative analysis, such as in high-performance liquid chromatography (HPLC) and other analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Caffeine, by Merck Group (3 mentions)
4 cqa, by Merck Group (3 mentions)
5 cqa, by Merck Group (2 mentions)
3 o caffeoylquinic acid, by Merck Group (2 mentions)
5 o caffeoylquinic acid, by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Merck Group (1 mentions)
5 cqa, by Carl Roth (1 mentions)
Milli q system, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
Mangiferin, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Trigonelline, by Merck Group (1 mentions)
Neochlorogenic acid, by Merck Group (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Milli q water system, by Merck Group (1 mentions)
4 o caffeoylquinic acid, by Merck Group (1 mentions)
4 protocols using 3 cqa
1
Analytical Characterization of Chlorogenic Acids
2
Quantification of Caffeine and Polyphenols
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Ultra-high purity water was obtained by filtration using a Milli-Q system from Millipore (Bedford, MA, USA). LC-MS quality acetonitrile, formic acid (FA) and trifluoroacetic acid (TFA) were purchased from Fisher Scientific (Waltham, MA, USA), while caffeine (99%), 3-CQA (>99%), 4-CQA (>99%), 5-CQA (>99%) and mangiferin (>99%) were purchased from Sigma-Aldrich (Steinheim, Germany).
3
Analytical Standards for Chlorogenic Acids
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Analytical standards of chlorogenic acid (5-CQA, CAS: 327-97-9, 5-O-caffeoylquinic acid), neochlorogenic acid (3-CQA, CAS: 906-33-2, 3-O-caffeoylquinic acid), caffeine (CAS: 58-08-2), and trigonelline (CAS: 535-83-1) were purchased from Sigma-Aldrich, Fluka, ChromaDex. All standards used were of analytical grade (≥ 99% purity). The 5-CQA standard was used to quantify both 5-CQA and 4-CQA. Mobile phase was prepared by diluting formic acid (CAS: 64-18-6, Fisher Scientific, LC–MS purity) with ultrapure water (Direct-Q system, resistivity below 18 MΩ cm) to a concentration of 0.1% (w/w) and the pH was adjusted to 2.4. The solution was filtered under a vacuum system through a 0.45 µm filter. The second part of the mobile phase was methanol (CAS: 67-56-1, POCH, HPLC grade). Stock solutions of 1000 mg/l were prepared by diluting each analysed compound in HPLC grade methanol. These stock solutions were diluted in methanol to reach intermediate concentrations. All solutions were stored in the refrigerator at 4 °C.
4
Quantification of Caffeoylquinic Acids in Juices
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Standards of 5-O-caffeoylquinic acid (chlorogenic acid; 5-CQA), 4-O-caffeoylquinic acid (cryptochlorogenic acid; 4-CQA) and 3-O-caffeoylquinic acid (neochlorogenic acid; 3-CQA) were purchased from Sigma-Aldrich Canada Ltd. (Oakville, ON, Canada). All standards were at least 95% in purity. HPLC grade methanol was obtained from Fisher Scientific (Ottawa, ON, Canada). The water used throughout this study was from a Millipore Milli-Q water system (Millipore Corp., Milford, MA, USA). Commercial apple and pear juice concentrates were from China and the United States, respectively.
Stock solutions of the caffeoylquinic acids at a concentration of 1.0 mM were prepared by dissolving the standards in 50% (v:v) aqueous methanol. The standards were then syringe filtered (nylon, 0.2 μm pore size, 13 mm diameter; Chromatographic Specialties, Brockville, ON, Canada) and stored at -20°C until use.
Stock solutions of the caffeoylquinic acids at a concentration of 1.0 mM were prepared by dissolving the standards in 50% (v:v) aqueous methanol. The standards were then syringe filtered (nylon, 0.2 μm pore size, 13 mm diameter; Chromatographic Specialties, Brockville, ON, Canada) and stored at -20°C until use.
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