Mcf 7 cell line

GnRH receptor-overexpressing human mammary adenocarcinoma (MCF-7) and prostate cancer (LNCaP) cell lines were obtained from the National Center for Cell Science (Pune, India). Cell culture conditions were maintained according to the requirement of the respective cell lines. The MCF-7 cell line was maintained in DMEM with 10% FBS (Himedia) and 1% penicillin/streptomycin (200 units of penicillin and 200 µg of streptomycin sulfate per milliliter of DMEM; Himedia). LNCaP cell line was maintained at 37°C in a humidified CO₂ (5%) incubator using RPMI-1640 media supplemented with 10% FBS and penicillin/streptomycin.

Silk cocoons
of B. mori (silkworm) were procured
from Central Silk Board, Dehradun, Uttarakhand, India. Poly(vinyl
alcohol) (PVA) (HiMedia, cat. no. GRM6170), sodium carbonate (HiMedia,
cat.no. GRM851), sodium bicarbonate (HiMedia, cat. no. MB045), lithium
bromide (SRL, cat. no. 31665), dialysis membrane (MWCO 12 kDa, HiMedia,
cat. no. LA401), ethanol (CSS, cat.no. 1170), glutaraldehyde (25%,
for synthesis, LOBA CHEMIE Pvt. Ltd., cat no. 03965), and formic acid,
98% AR (LOBA CHEMIE Pvt. Ltd., cat no. 00153) were purchased and used
as received. MCF-7 cell line (breast cancer cell line) was obtained
from NCCS, Pune, and cultured in Dulbecco’s modified eagle
medium (DMEM) (HiMedia, cat.no. AT007) containing 10% fetal bovine
serum (FBS, Gibco, cat. no. 10270–106) and 1% penicillin–streptomycin
(Gibco, cat. no. 10378-016). For cell culture experiments, Dulbecco’s
phosphate-buffered saline (DPBS, Ca²⁺- and Mg²⁺-free, Sigma-Aldrich, cat.no. D5652), trypsin-ethylenediaminetetraacetic
acid (EDTA) (0.25%, Sigma-Aldrich, cat. no. 25200-070), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, Amresco, cat.no. 0793), Hoechst 33342 (Sigma-Aldrich, cat.
no. 14533), and Rhodamine 123 (Sigma-Aldrich, cat. no. R8004) were
used.