Mef2 gal4

Fly stocks were obtained from the Bloomington Stock Center (BDSC) and the Vienna Drosophila Resource Center (v): tubP-Gal4 (BDSC5138), elav-Gal4 (BDSC458), Mef2-Gal4, UAS-tdTomato/TM6B (donated by Olaf Vef), Pdf-Gal4 (BDSC6899), UAS-CG3798/Nmda1-RNAi (#1 v28365, #2 BDSC28361), UAS-CG3814/Lfg-RNAi (#1 v4671, #2 BDSC33354), UAS-CG2076-RNAi (#1 v50221, #2 BDSC64564), UAS-CG1287-RNAi (#1 v12127), UAS-CG7188-RNAi (#1 v3235, #2 v37108), UAS-Always early-RNAi as RNAi control (v13673), UAS-erGAP3 (donated by M. Teresa Alonso), UAS-4mtGCaMP3, UAS-RFP (donated by Ronald Davis). The targeting sequence of RNAi strains are listed in Table 1. Flies were raised at 25°C and fed with standard food if not otherwise stated.

Drosophila flies were maintained at 24 °C, 60% relative humidity on a 12 h light: 12 h dark cycle and fed on a standard medium [23 (link)]. Wild-type w¹¹¹⁸ (+/+) (BDSC 5905), transgenic foxo^H [UAS foxo (BDSC 9575)] and foxo^L [UAS foxo (BDSC 42221)] lines, along with flies expressing the muscle-specific (high transgene expression) Mef2^Gal4 (BDSC 27390) driver were obtained from the Bloomington Drosophila Stock Center (University of Indiana, Bloomington, IN, USA). The muscle-specific (weak expression) driver Mhc^Gal4 was kindly provided by Dr. Orso (University of Padova, Padova, Italy). The cncC knockdown (UAS cncC^RNAi), the cncC overexpressing (UAS cncC) line and the Tub^Gal4 GeneSwitch (tubGSGal4) driver were a gift from Prof. Bohmann (University of Rochester, New York, NY, USA); the conditional driver Tub^Gal4 is ubiquitously activated upon dietary administration of 320 μΜ RU486 (#M8046, Mifepristone, Merck KGaA, Darmstadt, Germany). Cardiomyocyte-specific tinC.Δ4^Gal4 driver was donated by Prof. Frasch (Friedrich Alexander University, Erlangen-Nuremberg, Germany).