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Clone 1d3

Manufactured by BD
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Sourced in United Kingdom, United States

The Clone 1D3 is a laboratory equipment product designed for cell culture applications. It functions as a cell culture incubator, providing a controlled environment for the growth and maintenance of cells.

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Lab products found in correlation

Clone 145 2c11, by BD (2 mentions) Anti cd8 fitc, by BD (1 mentions) Guava easycyte, by Merck Group (1 mentions) Anti cd11b pe, by BD (1 mentions) Clone 53 6, by BD (1 mentions) Clone m1 70, by BD (1 mentions) Anti cd4 fitc, by BD (1 mentions) Anti cd3 pe, by BD (1 mentions) Anti cd19 pe, by BD (1 mentions) Mhc 2 clone m5 114, by Thermo Fisher Scientific (1 mentions) Cd3 clone 17a2, by BD (1 mentions) Cd11c clone n418, by Bio-Rad (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Il 23, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Cxcl1, by R&D Systems (1 mentions) Facscalibur, by BD (1 mentions) Cd44 clone im7, by BioLegend (1 mentions) Cd25 clone pc61, by BioLegend (1 mentions)

Close spelling variants of this product

CD19 FITC (clone 1D3, PE-conjugated anti-CD19 (clone 1D3, CD19-PE (clone 1D3, Anti-CD19 (clone 1D3, Anti-CD19 PerCP/Cy 5.5 (clone 1D3; CD19 (clone 1D3, APC rat anti-mouse CD19 clone 1D3 FITC-conjugatedanti-mouse CD19 (clone 1D3) Anti-mouse CD19-APC (clone 1D3, Anti-CD19 APC-H7 (clone 1D3;

5 protocols using clone 1d3

1

Murine Splenocyte Immunophenotyping by Flow Cytometry

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Mice spleen cell suspensions were prepared as reported by Da Silva et al.24 The concentration of each cell suspension was determined using a Neubauer chamber. Subsequently, 5 × 105 cells from each mouse were incubated with anti‐CD4 FITC (10 μg/ml; clone H129.19; BD Biosciences), anti‐CD8 FITC (10 μg/ml; clone 53–6.7; BD Biosciences), anti‐CD3 PE (10 μg/ml; clone 145‐2C11; BD Biosciences), anti‐CD11b PE (10 μg/ml; clone M1/70; BD Biosciences), or anti‐CD19 PE (10 μg/ml; clone 1D3; BD Biosciences) antibodies for 45 min at 4 °C. Cells were then washed with phosphate‐buffered saline (PBS) and resuspended in fresh PBS containing 0.5% (w/v) formaldehyde. An IgG isotype control antibody was used to calibrate the flow cytometry analysis. The frequency of CD4+ T cells, CD8+ T cells, CD 11b cells, and B cells were determined by flow cytometry (Guava easyCyte; Guava Technologies).
Miguel C.B., da silva T.A., Rodrigues W.F., Oliveira‐Brito P.K., Roque‐Barreira M.C, & Lazo‐Chica J.E. (2021). Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi. FASEB BioAdvances, 3(5), 295-304.
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2

Coculture of CAR-T and Lymphoma Cells

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For coculture experiments, anti-CD19 CAR-T cells were cocultured with a CD19+ PPMBC lymphoma cell line for 48 hours. Subsequently, cell counts were analyzed by flow cytometry after staining for CD3 (PE, BioLegend, clone 145-2C11) and CD19 (V500, BD Biosciences, Clone 1D3) after gating for Zombie-negative live cells.
Flümann R., Hansen J., Pelzer B.W., Nieper P., Lohmann T., Kisis I., Riet T., Kohlhas V., Nguyen P.H., Peifer M., Abedpour N., Bosco G., Thomas R.K., Kochanek M., Knüfer J., Jonigkeit L., Beleggia F., Holzem A., Büttner R., Lohneis P., Meinel J., Ortmann M., Persigehl T., Hallek M., Calado D.P., Chmielewski M., Klein S., Göthert J.R., Chapuy B., Zevnik B., Wunderlich F.T., von Tresckow B., Jachimowicz R.D., Melnick A.M., Reinhardt H.C, & Knittel G. (2022). Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention Strategies. Blood Cancer Discovery, 4(1), 78-97.
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3

Phenotypic and Functional Analysis of Mouse Dendritic Cells

Cited 1 time
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In flow cytometry (BD FACS Calibur, New Jersey, USA) the following monoclonal antibodies were used for cell staining: CD3 (clone 17A2, rat monoclonal, diluted 1:100, BD Biosciences, New Jersey, USA), CD11c (clone N418, hamster monoclonal, diluted 1:100, ABD Serotec, Kidlington, UK), CD19 (clone 1D3, rat monoclonal, diluted 1:100, BD Biosciences), CD40 (clone FGK45.5, rat monoclonal, diluted 1:40, Miltenyi), CD86 (clone PO3.1, rat monoclonal, diluted 1:80, eBioscience), CD80 (clone 16-10A1, rat monoclonal, diluted 1:400, Biolegend, Fell, Germany), MHCII (clone M5/114.15.2, rat monoclonal, diluted 1:800, eBioscience). All used antibodies have been validated by others [22 (link)]. The cytokines IL-1β, IL-4, IL-6, IL-10, IL-12, IL-17, IL-21, IL-23, TNF-α, IFN-γ (all eBioscience) and CXCL-1 (R&D systems, Minneapolis, USA) were measured in supernatants of bone marrow derived DC culture and MLR culture by using ELISA kits according to the manufacturer’s instructions.
A protein-based mouse cytokine/chemokine antibody array (Ray Biotech Inc, Norcross, USA) was performed on DC culture supernatant for comparative analysis of levels of a broad panel of 120 chemokine, cytokine and growthfactors in pooled supernatants of different types of DC cultures.
Kouwenberg M., Jacobs C.W., van der Vlag J, & Hilbrands L.B. (2016). Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype. PLoS ONE, 11(8), e0159986.
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4

Murine Thymocyte and Splenocyte Analysis

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Single-cell suspensions from thymi and spleens of 3- to 11-week-old mice were prepared by disrupting tissue and passing through a 70 μm-cell strainer (Falcon; Thermo Fisher Scientific). Cells were stained with the appropriate fluorochrome-labeled mAbs and analyzed on an LSRFortessa (BD Biosciences, San Jose, Calif). Antibodies were purchased from eBioscience (San Diego, Calif), unless otherwise indicated. Splenocyte and thymocyte subsets were defined by using fluorescently labeled antibodies against CD3 (clone145-2C11), CD4 (clone RM4-4), CD8 (clone 53–6.7), and B220 (clone RA3-6B2). Lineage-negative cells were identified by excluding cells stained with a single fluorochrome-labeled cocktail of phycoerythrin–labeled CD19 (BD, clone 1D3), CD3 (clone 145-2C11), CD4 (clone GK1.5), CD8 (clone 53–6.7), CD11b (clone M1/70), CD11c (clone N418), Ly6 (clone HK1.4), NK1.1 (clone PK136), T-cell receptor γδ (BD, clone GL3), and Ter-119 (clone Ter119)to distinguish thymocyte progenitors. Cells were subsequently stained with fluorescently labeled mAbs to CD44 (clone IM-7) and CD25 (clone PC61; BioLegend, San Diego, Calif).
Platt C.D., Chou J., Houlihan P., Badran Y.R., Kumar L., Bainter W., Poliani P.L., Perez C.J., Dent S.Y., Clapham D.E., Benavides F, & Geha R.S. (2017). Leucine-rich repeat containing 8A (LRRC8A)–dependent volume-regulated anion channel activity is dispensable for T-cell development and function. The Journal of allergy and clinical immunology, 140(6), 1651-1659.e1.
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5

Phenotyping Immune Cell Subsets in Murine Bone Marrow and Thymus

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Bone marrow (BM) cells were obtained by flushing femurs and/or humerus with PBS, and erythrocytes were lysed. Thymi were pressed through 70 µm cell strainers to obtain single-cell suspensions. Cellularity was determined using an automated cell counter (Sysmex, Noderstedt, Germany). Purified rat anti-mouse CD16/32 (BD Biosciences, Franklin Lakes, NJ, USA) was added to block antibodies from binding to Fc receptors, prior staining of surface markers. Cells were stained with the following fluorochrome-conjugated antibodies; antimouse CD8a PE (clone 53-6.7, Biolegend, San Diego, CA, USA), anti-mouse CD4 BD Horizon V450 (clone RM4-5, BD), anti-mouse CD117/ckit APC (clone 2B8, Biolegend), anti-mouse CD45R/B220 PerCP (clone RA3-6B2, Biolegend), anti-mouse CD19 BD Horizon V450 (clone 1D3, BD), anti-mouse IgM PE (clone 1B4B1, Southern Biotechnology, Birmingham, AL, USA) and anti-mouse CD93 PE-Cy7 (clone AA4.1, Biolegend). Fluorescenceminus-one (FMO)-stained samples were used as controls. Data were acquired on a BD FACS Canto II and analyzed using Flow Jo 8.8.6 (Three Star, Ashland, USA).
Andersson A., Törnqvist A.E., Moverare-Skrtic S., Bernardi A.I., Farman H.H., Chambon P., Engdahl C., Lagerquist M.K., Windahl S.H., Carlsten H., Ohlsson C, & Islander U. (2018). Roles of activating functions 1 and 2 of estrogen receptor α in lymphopoiesis. The Journal of endocrinology, 236(2).
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