Ultraflex 3

Biomedical grade G5 PAMAM dendrimer was purchased from Dendritech Inc. and purified using rp-HPLC to give a molecular weight fraction free of trailing generations (G1–G4) as well as G5 dimers and higher oligomers.^{28 (link)} Trifluoroacetic acid, HPLC grade water, GE PD-10 Sephadex columns, and HPLC grade acetonitrile were purchased from Fisher-Scientific and used as received. 5-carboxy tetramethylrhodamine succinimydyl ester (TAMRA) was purchased from Life Technologies. A 500 MHz Varian NMR instrument was used for all ¹H and ¹⁹F NMR measurements. All MALDI-TOF MS measurements were performed on a Bruker Ultraflex III with sinapinic acid matrix (Sigma Aldrich) and sodium trifluoroacetate (Fischer Scientific) salt sample preparation. Serum-free DMEM (SFM) from life technologies was employed for cell culture of HEK293A cells, which were obtained from ATCC. Complete medium was made by adding 50 mL of fetal bovine serum (FBS) and 5 mL 100× of penicillin streptomycin to 500 mL of SFM.

Biomedical grade G3 PAMAM dendrimer with ethylenediamine core was purchased from Dendritech Inc. and purified using rp-HPLC to give a molecular weight fraction free of trailing generations, dimers and higher oligomers.²³ Trifluoroacetic acid, HPLC grade water, GE PD-10 sephadex column, and HPLC grade acetonitrile were purchased from Fisher-Scientific and used as received. Oregon Green 488 was purchased from Life Technologies. A 500 MHz Varian NMR instrument was used for all ¹H NMR measurements. All MALDI-TOF MS measurements were performed on a Bruker Ultraflex III with sinapinic acid matrix (Sigma-Aldrich) and sodium trifluoroacetate (Fischer Scientific) salt sample preparation. Serum-free DMEM (SFM) and RPMI from life technologies was employed for cell culture of HEK 293A cells, which were obtained from Life Technologies. Complete media was made by adding 50 mL of fetal bovine serum (FBS) and 5 mL of 100× of penicillin–streptomycin to 500 mL of SFM.