sEVs were isolated using miRCURY Exosome Kits (from Cell/Urine/CSF; Qiagen, Valencia, CA) following the manufacturer’s protocol. Briefly, 400μL of the precipitation buffer B was added to 1 mL of CSF samples and mixed well at 4 °C for 60 minutes, followed by centrifugation at 10,000 g for 30 minutes at 25 °C. The sEVs were harvested by removing the supernatants. The sEVs were resuspended in 100 μL of resuspension buffer, and were then ready for further analysis. Then, sEVs were lysed using 1% Triton X-100 lysis buffer (Beyotime, P0013) that contained protease and phosphatase inhibitor cocktails. The lysates were stored at −80°C.
Triton x 100 lysis buffer
Manufactured by Beyotime
Sourced in China
Triton X-100 lysis buffer is a non-ionic detergent solution used to extract proteins from cells and tissues. It is commonly used in molecular biology and biochemistry applications to solubilize cell membranes and release cellular contents for further analysis or purification.
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Lab products found in correlation
2 protocols using triton x 100 lysis buffer
1
Isolation and Lysis of Small Extracellular Vesicles from Cerebrospinal Fluid
2
Quantification of GE-T Uptake in Cell Lines
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The parental and drug-resistant cell lines were seeded into 6-well plates overnight. The cells were incubated with tritium-labeled GE (GE-T) in the presence or absence of MA for 2 h, 24 h, 48 h, or 72 h, respectively, at 37°C containing 5% CO2. Then, the cells were lysed by Triton X-100 lysis buffer (Beyotime, Shanghai, China). The radioactivity of the total lysis product was measured by a Tri-Card 4910 liquid scintillation analyzer (PerkinElmer, Waltham, MA, United States).
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