To analyze cellular biodistribution of P-Tofa, Alexa Flour® 647 labeled P-Tofa (P-Tofa-Alexa 647 200 mg polymer/kg mouse) was mixed with P-Tofa to a total Tofa equivalent dose of 210 mg/kg and i.v. injected to mice with DSS exposure (n = 3/time point) on day 7 post-induction. Mice were euthanized 1 and 3 days after the administration of P-Tofa-Alexa 647. Blood and major organs (liver, spleen, kidney, colon, and mesenchymal lymph nodes) were collected and processed for flow cytometry analysis. Blood cells were marked by the following antibodies: BF650-labeled anti-mouse F4/80, BV711-labeled anti-mouse CD11b, BV786-labeled anti-mouse CD3e, and PE-Cy7-labeled CD19; BV421-labeled anti-mouse CD11c, APC-eFluor 780-labeled anti-mouse Ly-6G (BD Biosciences), PE/Dazzle™ 594 anti-mouse CD45 (Biolegend). The cells were analyzed using LSR II Green flow cytometer (BD Biosciences) and FlowJo software (Treestar, Inc., San Carlos, CA).
Bv711 labeled anti mouse cd11b
Manufactured by BD
BV711-labeled anti-mouse CD11b is a fluorescently-conjugated antibody that specifically binds to the CD11b surface antigen expressed on mouse myeloid cells. It can be used to identify and analyze these cell populations in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bv711 labeled anti mouse cd11b
1
Biodistribution of Labeled P-Tofa in Mice
2
Comprehensive Multi-Organ Immune Profiling
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After the proteinuria was established,
NZB/W F1 mice (n = 3 for each time point) and NZW
mice (n = 5 for each time point) were given ZSJ-0228-AF647
(Alexa Fluor 647 dose of 300 nmol/kg, dexamethasone equivalent dose
of 28 mg/kg) via tail vein injection. The animals
were euthanized and perfused at 1 and 4 days postadministration. Blood,
bone marrow, heart, lungs, kidneys, liver, and spleen were harvested
and processed to obtain single-cell suspensions. These cells were
marked by the following antibodies: BV711-labeled anti-mouse CD11b,
BV786-labeled anti-mouse CD3e, BUV395-labeled anti-mouse NK1.1, PerCP-Cy5.5
labeled-CD146, PE-Cy7-labeled CD19, BV510-labeled anti-mouse CD11c,
APC-eFluor 780-labeled anti-mouse Ly-6G (BD Biosciences), APC-labeled
anti-mouse F4/80 and PE-labeled anti-mouse CD326 (eBioscience Inc.).
The cells were then analyzed using a BD LSR II Green flow cytometer
with FlowJo software.
NZB/W F1 mice (n = 3 for each time point) and NZW
mice (n = 5 for each time point) were given ZSJ-0228-AF647
(Alexa Fluor 647 dose of 300 nmol/kg, dexamethasone equivalent dose
of 28 mg/kg) via tail vein injection. The animals
were euthanized and perfused at 1 and 4 days postadministration. Blood,
bone marrow, heart, lungs, kidneys, liver, and spleen were harvested
and processed to obtain single-cell suspensions. These cells were
marked by the following antibodies: BV711-labeled anti-mouse CD11b,
BV786-labeled anti-mouse CD3e, BUV395-labeled anti-mouse NK1.1, PerCP-Cy5.5
labeled-CD146, PE-Cy7-labeled CD19, BV510-labeled anti-mouse CD11c,
APC-eFluor 780-labeled anti-mouse Ly-6G (BD Biosciences), APC-labeled
anti-mouse F4/80 and PE-labeled anti-mouse CD326 (eBioscience Inc.).
The cells were then analyzed using a BD LSR II Green flow cytometer
with FlowJo software.
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