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Propidum iodide

Manufactured by Beyotime
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Sourced in China

Propidium iodide is a fluorescent dye used in molecular biology for nucleic acid staining. It binds to DNA by intercalating between base pairs. This property allows it to be used as a counterstain in flow cytometry, fluorescence microscopy, and other nucleic acid detection techniques.

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Lab products found in correlation

Annexin 5 fitc, by Beyotime (3 mentions) Calibur flow cytometry, by BD (2 mentions) Cellquest software, by BD (2 mentions) Propidium iodide, by BD (1 mentions)

3 protocols using propidum iodide

1

Cell Cycle and Apoptosis Analysis

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Twenty-four hour after transfection, cells were trypsinized and fixed with 70% ethanol for 30 min on ice. RNA was degraded by incubation with 20 mg/ml RNase (Sigma) for 1 h at 37°C. For the cell cycle analysis, cells were stained with propidium iodide at room temperature for 30 min, and then were analyzed by Calibur flow cytometry (BD Biosciences, Franklin Lakers, USA). For cell apoptosis analysis, cells were stained with FITC-Annexin V and propidum iodide (Beyotime, Beijing, China) and then were analyzed by Calibur flow cytometry (BD Biosciences) equipped with CellQuest software (BD Biosciences).
Cai H., Yao J., An Y., Chen X., Chen W., Wu D., Luo B., Yang Y., Jiang Y., Sun D, & He X. (2017). LncRNA HOTAIR acts as competing endogenous RNA to control the expression of Notch3 via sponging miR-613 in pancreatic cancer. Oncotarget, 8(20), 32905-32917.
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2

Cell Apoptosis Analysis by Flow Cytometry

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Twenty-four hour after transfection, cells were trypsinized and fixed with 70% ethanol for 30 min on ice. RNA was degraded by incubation with 20 mg/ml RNase (Sigma) for 1 h at 37°C. Cells were stained with FITC-Annexin V and propidum iodide (Beyotime, Beijing, China) and then were analyzed by Calibur flow cytometry (BD Biosciences, Franklin Lakes, USA) equipped with CellQuest software (BD Biosciences).
Cai H., An Y., Chen X., Sun D., Chen T., Peng Y., Zhu F., Jiang Y, & He X. (2016). Epigenetic inhibition of miR-663b by long non-coding RNA HOTAIR promotes pancreatic cancer cell proliferation via up-regulation of insulin-like growth factor 2. Oncotarget, 7(52), 86857-86870.
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3

Annexin V-FITC Apoptosis Assay

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For cell apoptosis analysis, cells at 2 × 105 were washed in ice-cold PBS. Then cells were stained with FITC–annexin V and propidum iodide (Beyotime, Beijing, P.R. China) following the manufacturer’s instructions. Cell apoptosis was analyzed in a flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Jiang C., Yang Y., Yang Y., Guo L., Huang J., Liu X., Wu C, & Zou J. (2018). Long Noncoding RNA (lncRNA) HOTAIR Affects Tumorigenesis and Metastasis of Non-Small Cell Lung Cancer by Upregulating miR-613. Oncology Research, 26(5), 725-734.
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