Anti cd49f goh3

Manufactured by Thermo Fisher Scientific

Anti-CD49f (GoH3) is a monoclonal antibody that recognizes the CD49f (alpha 6 integrin subunit) antigen. CD49f is a cell surface molecule expressed on various cell types, including stem cells, epithelial cells, and certain immune cells. This antibody can be used for the identification and isolation of CD49f-positive cells in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd4 rm4 5, by Thermo Fisher Scientific (3 mentions) Prolong gold, by Thermo Fisher Scientific (3 mentions) Anti gfp a21311, by Thermo Fisher Scientific (2 mentions) Hc pl apo 40x 1.3 oil, by Leica (2 mentions) Tcs sp8 confocal microscope, by Leica (2 mentions) Anti cd8α clone 53 6, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Anti tcrβ h57 597, by Thermo Fisher Scientific (1 mentions) Anti cd8α 53 6, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Phosphate buffered saline (pbs), by Electron Microscopy Sciences (1 mentions) Anti cd45.1 a20, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde solution, by Electron Microscopy Sciences (1 mentions)

3 protocols using anti cd49f goh3

Immunofluorescent Staining of Ear Pinnae

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ear pinnae were split with forceps, fixed in 1% paraformaldehyde (Electron Microscopy Sciences) overnight at 4°C, and blocked in PBS containing 1% BSA and 0.25% Triton X-100 for 2 h at room temperature. Tissues were first stained with anti-CD4 (RM4–5, eBioscience), anti-CD8α (53–6.7, eBioscience), anti-CD49f (GoH3, eBioscience), anti-GFP (A21311, Life Technologies), and anti-TCRβ (H57–597, eBioscience) antibodies overnight at 4°C, washed three times with PBS, and then stained with DAPI (Sigma-Aldrich) overnight before being mounted with ProLong Gold (Invitrogen). Ear pinnae images were captured on a Leica TCS SP8 confocal microscope equipped with HyD and PMT detectors and a 40X oil objective (HC PL APO 40X/1.3 oil). Images were analyzed using Imaris (Bitplane).

Constantinides M.G., Link V.M., Tamoutounour S., Wong A.C., Perez-Chaparro P.J., Han S.J., Chen Y.E., Li K., Farhat S., Weckel A., Krishnamurthy S.R., Vujkovic-Cvijin I., Linehan J.L., Bouladoux N., Merrill E.D., Roy S., Cua D.J., Adams E.J., Bhandoola A., Scharschmidt T.C., Aubé J., Fischbach M.A, & Belkaid Y. (2019). MAIT cells are imprinted by the microbiota in early life and promote tissue repair. Science (New York, N.Y.), 366(6464), eaax6624.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Murine Ear Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ear pinnae were split with forceps, fixed in 1% paraformaldehyde in PBS (Electron Microscopy Sciences) overnight at 4°C and blocked in 1% BSA + 0.25% Triton X in PBS for 2 hours at room temperature. Tissues were first stained with anti-CD4 (RM4–5, eBioscience), anti-CD8α (clone 53–6.7, eBioscience), anti-CD45.1 (A20, eBioscience), anti-CD49f (GoH3, eBioscience) and/or anti-GFP (A21311, Life Technologies) antibodies overnight at 4°C, washed three time with PBS and then stained with 4,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) overnight before being mounted with ProLong Gold (Molecular Probes) antifade reagent. Ear pinnae images were captured on a Leica TCS SP8 confocal microscope equipped with HyD and PMT detectors and a 40× oil objective (HC PL APO 40X/1.3 oil). Images were analyzed using Imaris software (Bitplane).

Harrison O.J., Linehan J.L., Shih H.Y., Bouladoux N., Han S.J., Smelkinson M., Sen S.K., Byrd A.L., Enamorado M., Yao C., Tamoutounour S., Van Laethem F., Hurabielle C., Collins N., Paun A., Salcedo R., O’Shea J.J, & Belkaid Y. (2018). Commensal-specific T cell plasticity promotes rapid tissue adaptation to injury. Science (New York, N.Y.), 363(6422), eaat6280.

+ Open protocol

+ Expand

Ear Pinnae Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ear pinnae were split with forceps, fixed in 1% paraformaldehyde solution (Electron Microscopy Sciences) overnight at 4°C and blocked in 1% BSA, 0.25% Triton X blocking buffer for 2 hours at room temperature. Tissues were first stained with anti-CD4 (RM4-5, eBioscience), anti-CD8α (clone 53- 6.7, eBioscience), anti-CD49f (GoH3, eBioscience) antibodies overnight at 4°C, washed three times with PBS and then mounted with ProLong Gold (Molecular Probes) antifade reagent. Ear pinnae images were captured on a Leica TCS SP8 confocal microscope with a 40X oil objective (HC PL APO 40X/1.3 oil). Images were analyzed using Imaris Bitplane software.

Lima-Junior D.S., Krishnamurthy S.R., Bouladoux N., Collins N., Han S.J., Chen E.Y., Constantinides M.G., Link V.M., Lim A.I., Enamorado M., Cataisson C., Gil L., Rao I., Farley T.K., Koroleva G., Attig J., Yuspa S.H., Fischbach M.A., Kassiotis G, & Belkaid Y. (2021). Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota. Cell, 184(14), 3794-3811.e19.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!