Mouse β actin antibody

The DDIT4 (rabbit) antibody (#10,638–1-AP, 1:1000 dilution) and β-actin (mouse) antibody (#66,009–1-Ig, 1:5000 dilution) were obtained from Proteintech (Chicago, IL, USA). Anti-NF-κB p65 (#6956, 1:1000 dilution) and anti-phospho-p65 (#3033, 1:1000 dilution) were obtained from Cell Signaling Technology (Danvers, MA, USA). Cy3-labeled goat anti-mouse (#A0521, 1:200 dilution) and FITC-labeled goat anti-rabbit antibodies (#A0562, 1:200 dilution) and DAPI were obtained from Beyotime Institute of Biotechnology (China). The DDIT4-AS1 antisense oligonucleotides (ASO) and the control ASO were purchased from Integrated Biotech Solutions Co., Ltd. (Shanghai, China); the sequences are listed in Table S2. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 plasmid pYSY-spCas9-sgRNA-Puro was obtained from YSY Biotech (Nanjing, China). The transfection reagent jetPRIME was purchased from Polyplus Transfection (Illkirch, France). The RNA polymerase II transcription inhibitor α-amanitin was purchased from Medchem Express (Princeton, NJ, USA). RNAse A + T cocktail was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The super electrochemiluminescence (ECL) kit was obtained from US Everbright Inc. (Suzhou, China).

2,3,5-Triphenyltetrazolium chloride (TTC) and 5-bromo-2′-deoxyuridine (BrdU) were purchased from Sigma Chemical Co. Tanshinol, ferulic acid (FA), baicalin, protocatechuic acid, rosmarinic acid, salvianolic acid B, hydroxysafflor yellow A and 9′-methyl lithospermate B were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Rabbit monoclonal Bax antibody (#14796s, 1:1000), rabbit monoclonal IL-1β antibody (#12426s, 1:1000), rabbit monoclonal IL-6 antibody (#12912p, 1:1000) were purchased from Cell Signal Technology. Mouse monoclonal TNF antibody (#60291-1-Ig, 1:500), rabbit polyclonal BDNF antibody (#25699-1-AP, 1:200), and mouse β-actin antibody (#60008-1-Ig, 1:4000) were purchased from Proteintech Group, Inc., and rabbit polyclonal Bcl-2 antibody (#bs-0032R, 1:100) was purchased from Bioss (Beijing).