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Ab279654

Manufactured by Abcam
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Sourced in United States, United Kingdom

This product is a pipette tip rack. It is designed to hold and organize pipette tips for laboratory use. The core function of this product is to provide a convenient and organized storage solution for pipette tips.

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Lab products found in correlation

Cd3 percp cy5, by BioLegend (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Cd8 pe, by BioLegend (1 mentions) Ab15580, by Abcam (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions) Cd80 pe, by BioLegend (1 mentions) Cd86 apc, by BioLegend (1 mentions) Cd11c fitc, by BioLegend (1 mentions) Ab191838, by Abcam (1 mentions) Ab185966, by Abcam (1 mentions) Ab47010, by Abcam (1 mentions) Ab128033, by Abcam (1 mentions) Pierce 660 protein assay, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab279654

1

PBA-PEG-PBA Nanocarrier Synthesis

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PBA-PEG-PBA was synthesized by our lab. Doxorubicin hydrochloride (DOX·HCl), ferrous chloride tetrahydrate (FeCl2·4H2O), adenosine triphosphate disodium (ATP) and benzoic acid (BA) were purchased from Aladdin (Shanghai, China). 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) was supplied from Sigma–Aldrich (USA). CD3-PerCP Cy5.5 (clone: 145-2C11), CD4-FITC (clone: GK1.5), CD8-PE (clone: 53–6.7), CD11c-FITC (clone: N418), CD80-PE (clone: 16-10A1), CD86-APC (clone: GL-1), CD11b-FITC (clone M1/70), CD206-PE (clone C068C2), F4/80-APC (clone BM8.1) were all purchased from Biolegend (USA). CRT primary antibody (ab92516), HMGB1 primary antibody (ab18256), HIF-1α primary antibody (ab279654) and ki-67 primary antibody (ab15580) were all purchased from Abcam (USA). IL-6, TNF-α, IFN-γ ELISA kit were purchased from Abcam (USA).
Zhang J., Sun X., Zhao X., Yang C., Shi M., Zhang B., Hu H., Qiao M., Chen D, & Zhao X. (2022). Combining immune checkpoint blockade with ATP-based immunogenic cell death amplifier for cancer chemo-immunotherapy. Acta Pharmaceutica Sinica. B, 12(9), 3694-3709.
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2

Western Blot Analysis of HIF-1α and Metabolic Regulators

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Cell lysates were prepared using RIPA buffer supplemented with sodium orthovanadate, sodium fluoride and phenyl methyl sulphonyl fluoride (1 mM each). Total protein was quantified using the Pierce 660 Protein Assay (Thermo Scientific, Waltham, MA, USA) and equal amounts of protein loaded on to SDS-PAGE gels. Western blotting was carried out using standard protocols. Blots were probed with primary antibodies for HIF1α (ab279654), SOX9 (ab185966), GLUT1 (ab128033), LDHA (ab47010), IDH2 (ab129180) and PGC1α (ab191838) (Abcam, Cambridge, UK).
Jain L., Bolam S.M., Monk A.P., Munro J.T., Chen E., Tamatea J., Dalbeth N, & Poulsen R.C. (2023). Differential Effects of Hypoxia versus Hyperoxia or Physoxia on Phenotype and Energy Metabolism in Human Chondrocytes from Osteoarthritic Compared to Macroscopically Normal Cartilage. International Journal of Molecular Sciences, 24(8), 7532.
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