Aers 500

Manufactured by Thermo Fisher Scientific

The AERS 500 is a laboratory instrument designed for automated external respiratory stimulation. It is capable of delivering controlled and programmable airflow and pressure to facilitate respiratory testing and research applications.

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Lab products found in correlation

Dionex ics 2100, by Thermo Fisher Scientific (1 mentions) Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions) As11 hc guard column, by Thermo Fisher Scientific (1 mentions) Dionex dx500, by Thermo Fisher Scientific (1 mentions) Rfc 30, by Thermo Fisher Scientific (1 mentions) Egc 3 koh, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

AERS 500 suppressor (3 citations) Dionex AERS 500 (2 citations)

5 protocols using aers 500

Quantification of Nitrite in E-Liquids

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Nitrite (CAS # 14797-65-0) in e-liquids was analyzed
using ion chromatography
(IC). The method was developed and adapted from the IC method reported
in the CORESTA technical report.³⁴ The
IC instrument consisted of a Thermo Scientific Dionex Ion Chromatography
ICS 5000 and AERS 500 electrolytically regenerated suppressor, AS
19 analytical column (4 mm × 250 mm), and AG 19 guard column
(4 mm × 50 mm; Sunnyvale, CA). The eluent consisted of potassium
hydroxide at gradient concentration ranges of 10–55 mM and
was generated with a potassium hydroxide eluent generator cartridge
(Thermo Scientific). ISO Guide 34-certified nitrite stock solution
(1000 ppm) was purchased from Thermo Scientific. The e-liquid sample
(0.2 g) was added to 10 mL of Type I water and mixed well. The samples
were then filtered and analyzed using IC with suppressed conductivity
detection. The calibration range was 0.01–1 μg/mL. The
LOQ for nitrite in e-liquid was 0.5 μg/g of e-liquid based on
the lowest calibration standard.

Jin X.C., Wagner K.A., Melvin M.S., Smith D.C., Pithawalla Y.B., Gardner W.P., Avery K.C, & Karles G.D. (2022). Influence of Nitrite on Formation of Tobacco-Specific Nitrosamines in Electronic Cigarette Liquids and Aerosols. Chemical Research in Toxicology, 35(5), 782-791.

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Nitrate Quantification in Oocyte Extracts

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Nitrate concentration in the oocyte extracts was quantified using a Dionex ICS-2100 anion exchange chromatography system (Thermo Scientific). The separation was done on a Dionex IonPac AG11-HC analytical column coupled to the AS11-HC guard column (Thermo Scientific). The columns were connected to a Dionex AERS 500 anion suppressor (Thermo Scientific). The analyses were performed under the following conditions: sample injection volume 4.8 μl, column temperature 30 °C, flow rate of 0.38 ml/min, isocratic eluent gradient using 30 mM KOH solution in QH₂O, suppressor current of 29 mA, and runtime of 15 min. The nitrate detection was done at 220 nm using a Dionex UltiMate 3000 (Thermo Scientific). QH2O water dilutions of Dionex Combined Seven Anion Standard (Thermo Scientific) were used to create a standard calibration curve. Accuracy and precision of the quantification was checked by including samples of potassium nitrate throughout the sequence.

Binenbaum J., Wulff N., Camut L., Kiradjiev K., Anfang M., Tal I., Vasuki H., Zhang Y., Sakvarelidze-Achard L., Davière J.M., Ripper D., Carrera E., Manasherova E., Yaakov S.B., Lazary S., Hua C., Novak V., Crocoll C., Weinstain R., Cohen H., Ragni L., Aharoni A., Band L.R., Achard P., Nour-Eldin H.H, & Shani E. (2023). Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis. Nature plants, 9(5), 785-802.

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Ion Chromatography of Anions

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The chromatographic instrument (Thermo Scientific Dionex DX-500) consisted of a gradient pump (Dionex GP50) with a flow of 0.25 mL/min, an autosampler (Dionex AS50) with 1000-µL-injection loop, a column thermostat set at 30 °C (Dionex Ultimate 3000 TCC-3000), an eluent generator (Dionex RFC-30) equipped with an eluent generator cartridge (Dionex EGC-III KOH), continuously regenerated trap column (Dionex CR-ATC), and a conductivity detector (Dionex CD-25). The entire flow path was metal free. For eluent suppression prior to conductivity detection, a Dionex AERS 500 was used at a current setting of 22 mA. Separation was performed on a Dionex IonPac AS20 column and guard column-set. Columns and suppressor were in the 2-mm format, and data acquisition and evaluation were done using the Dionex Chromeleon 6.70 chromatography software. The eluent (35 mmol/L KOH) was produced electrolytically in situ. Samples were injected with volumes between 500 and 1000 µL using partial loop injection for volumes below 1000 µL. The total runtime was 30 min for each sample.

Seiler M.A., Jensen D., Neist U., Deister U.K, & Schmitz F. (2016). Validation data for the determination of perchlorate in water using ion chromatography with suppressed conductivity detection. Environmental Sciences Europe, 28(1), 18.

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Quantifying Fucoidan Sulfate Content

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The sulfate content of fucoidans substrates as well as sulfate release after sulfatase treatment was analyzed by high performance anion exchange chromatography (HPAEC) using a Dionex ICS-5000 chromatography system equipped with a GP40 gradient pump and an ED40 electrochemical detector. The ions were separated on an HC-AS11 anion-exchange column (4 × 250 mm; Dionex) with accompanying HC-AG11 guard column (4 × 50 mm; Dionex). Elution was performed with 35 mM NaOH using an isocratic flow rate of 1.5 ml min⁻¹. Background signal and noise originating from the eluent was reduced using an anion self-regenerating suppressor (AERS-500, Dionex) with a current of 180 mA. Sulfate concentration was deduced from the signal intensity and calculated from a standard sulfate calibration curve using Na₂SO₄. The release of sulfate was related to the amount of sulfate present in the polysaccharide substrate, 33 ± 3% in S. mcclurei^{2 (link)} and 30 ± 2% in F. evanescens^{45 (link)}.

Mikkelsen M.D., Cao H.T., Roret T., Rhein-Knudsen N., Holck J., Tran V.T., Nguyen T.T., Tran V.H., Lezyk M.J., Muschiol J., Pham T.D., Czjzek M, & Meyer A.S. (2021). A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure. Scientific Reports, 11, 19523.

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Leaching Potential Assessment of PTEs from BA-S

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The leaching potential of PTEs from BA-S was evaluated with the one-batch leaching test, according to EN12457-2 [23] . The sample was mixed with ultrapure deionized water in a polyethylene bottle with a liquid-to-solid ratio of 10 l/kg. The mixture was placed for the duration of 24 h on a linear reciprocating shaker (Stuart SSL2). Afterwards, the leachates were filtered and acidified with 0.2 vol.% of the HNO 3 (69%, ACS reagent grade). These leachates were analyzed for elemental composition with ICP-OES (inductively coupled plasma-optical emission spectrometer; Spectroblue from Sysmex). The contents of chlorides and sulfates in the leachates were determined with ion-chromatography (Dionex 1100), which was equipped with an ion exchange column AS9-HS (2 x 250 mm). A Na 2 CO 3 (9 mM) solution with an isocratic flow of 0.25 ml/min was used as eluent. An electrolytically regenerated suppressor (Dionex AERS 500, 2 mm was used to measure suppressed conductivity for the detection of ions.

Alam Q., Schollbach K., Rijnders M., van Hoek C., van der Laan S, & Brouwers H.J.H. (2019). The immobilization of potentially toxic elements due to incineration and weathering of bottom ash fines. Journal of hazardous materials, 379.

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