Cell apoptosis was detected by Annexin V‐FITC Apoptosis Detection Kit (KeyGEN BioTECH, China) following the manufacturer's instruction and then analysed with CytoFLEX (Beckman Coulter, Brea , CA, USA). Cell cycle assay was carried out using PI/RNase Staining Kit (Dojindo) and FITC BrdU Flow Kit (BD Pharmigen, San Diego, CA, USA) in accordance with the manufacturer's instruction. Flow cytometry analysis was performed using CytoFLEX (Beckman Coulter).
Pi rnase staining kit
Manufactured by Dojindo Laboratories
Sourced in United States
The PI/RNase Staining Kit is a laboratory product designed for the detection and analysis of DNA content in cells. The kit utilizes propidium iodide (PI) as a fluorescent dye that binds to DNA, and RNase to remove RNA, enabling the measurement of DNA content specifically. This product is intended for use in flow cytometry and other applications requiring the determination of cellular DNA content.
Automatically generated - may contain errors
Lab products found in correlation
Fitc brdu flow kit, by BD (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Keygen Biotech (1 mentions)
Cell counting kit 8 cck 8, by Elabscience (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Elabscience (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Solarbio (1 mentions)
2 protocols using pi rnase staining kit
1
Apoptosis and Cell Cycle Analysis
2
Cytotoxic Effects of HA-SS-Geraniol in Prostate Cancer
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Geraniol was commercially bought from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). F12K medium, fetal bovine serum (FBS), and penicillin-streptomycin (PS) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China), Corning Inc. (Auckland, New Zealand), and Gibco, Thermo Fisher Scientific Inc., respectively. HA-SS-geraniol was provided by Dr. Duan’s lab at Henan University and synthesized as described previously.29 (link) The human PC-3 prostate cancer cell line was purchased from the Peking Union Medical College Cell Culture Center (1101HUM-PUMC000115) (Beijing, China). The Cell Counting Kit-8 (CCK-8) and Annexin V-FITC Apoptosis Detection Kit were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). The Cellstain® Rhodamine 123 (Rh-123) Kit was procured from Sigma-Aldrich, Merck Ltd. The PI/RNase Staining Kit was purchased from Dojindo Molecular Technologies, Inc.
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