Dimethylsulfoxide solution dmso

Rosewell Park Memorial Institute (RPMI) 1640 media (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) (Tico Europe, Amstelveen, The Netherlands), 1% penicillin-streptomycin (Nacalai Tesque, Kyoto, Japan), absolute ethanol (Systerm, Selangor, Malaysia), 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST-1) (Roche, Basel, Switzerland), dimethylsulfoxide solution (DMSO) (Sigma, St. Louis, MO, USA), trypan blue 0.4% (Sigma, St. Louis, MO, USA), phosphate buffer solution (PBS) (Medicago, Uppsala, Sweden), Vincristin (ChemFaces, Hubei, China), Annexin V-FITC/PI kit (BD Bioscience, East Rutherford, NJ, USA), RNase/PI stain (BD Bioscience, East Rutherford, NJ, USA), L-glutamate (Sigma, St. Louis, MO, USA), organotin(IV) dithiocarbamate compounds.

The effect of the glasses on the growth and proliferation of hiPSCs was evaluated using the standard colorimetric 3-(4, 5-dimethyl-2 thiazolyl) - 2,5-diphenyl- 2H-tetrazolium bromide (MTT) assay. For this purpose, the cells were seeded into 96-well cell culture plates (SPL Life sciences, Korea) at a concentration of 5 × 10³ cells/ well. After the incubation of 24 h, the culture medium of iPSCs was changed with the conditioned media (containing 4 mg/ ml of each glass), and cells were cultured in this condition for 1, 5, and 7 days. At the end of each time point, the MTT solution (5 mg/ml) (Sigma-Aldrich, USA) was added to the plates and incubated for another 4 h. Then all the cell culture medium was pulled out, and dimethyl sulfoxide solution (DMSO) (Sigma-Aldrich, USA) added to the plates. As a final point, optical density (OD) of each plate was measured by a spectrophotometer (Synergy HT, BioTek, USA) at 570 nm wavelength. The number of living cells was calculated using the following formula: