Immunofluorescence for NLRP3 and apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) was performed according to previous research (Feng et al., 2014 (link)). Platelets attached on a poly-L-lysine-coated coverslip were fixed with pre-cooled methanol for 20 min, washed twice with PBS (135 mM NaCl, 4.7 mM KCl, 10 mM Na2HPO4, 2 mM NaH2PO4, pH 7.4), and blocked with 10% fetal calf serum (FCS) in PBS. Then, platelets were incubated with goat anti-rat NLRP3 antibody (1:1000; Abcam) or rabbit anti-rat ASC antibody (1:500; Santa Cruz) for 30 min, washed twice with PBS, and labeled with corresponding Dlight 488 and Dlight 594 conjugated secondary antibodies (1:1000; Invitrogen) for 30 min respectively. Preparations were analyzed under a laser scanning confocal microscope (Fluo View FV1000; Olympus), and FV1000 operations software was used for recording.
Rabbit anti rat asc antibody
Manufactured by Santa Cruz Biotechnology
The Rabbit anti-rat ASC antibody is a research-use antibody that specifically binds to the ASC (Apoptosis-associated Speck-like Protein Containing a CARD) protein in rat samples. This antibody can be used to detect the presence and distribution of the ASC protein in rat-derived specimens through techniques such as Western blotting, immunohistochemistry, or immunocytochemistry.
Automatically generated - may contain errors
2 protocols using rabbit anti rat asc antibody
1
Immunofluorescence Imaging of NLRP3 and ASC
2
Immunofluorescence of NLRP3 and ASC in Platelets
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Immuno uorescence for NLRP3 and apoptosis-associated speck-like protein containing a caspaserecruitment domain (ASC) was performed according to previous research [18] . Platelets attached on a poly-L-lysine-coated coverslip were xed with pre-cooled methanol for 20 min, washed twice with PBS (135 mM NaCl, 4.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM NaH 2 PO 4 , pH 7.4), and blocked with 10% fetal calf serum (FCS) in PBS. Then, platelets were incubated with goat anti-rat NLRP3 antibody (1:1000, Abcam) or rabbit anti-rat ASC antibody (1:500, Santa Cruz) for 30 min, washed twice with PBS, and labeled with corresponding Dlight 488 and Dlight 594 conjugated secondary antibodies (1:1000, Invitrogen) for 30 min respectively. Preparations were analyzed on a laser scanning confocal microscope (Fluo View FV1000, Olympus), and FV1000 operations software was used for recording.
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