Terminal deoxynucleotidyl transferase mediated dutp nick end labeling tunel cell apoptosis detection kit

DSPE-PEG₂₀₀₀ was purchased from Avanti Polar Lipids, Inc. Poly-L-lysine was purchased from Sigma-Aldrich. Agarose was purchased from Biowest. N2200 was purchased from Derthon Optoelectronics Materials Science Technology Company (Shenzhen, China). Tetrahydrofuran (THF) was purchased from J&K. RIPA Lysis Buffer, protein microBCA assay kit, and LIVE/DEAD™ cell imaging kit were purchased from Thermo Fisher (Massachusetts, USA). CCK-8 assay, 4’,6-diamidino-2-phenylindole (DAPI), Rhodamine-phalloidin, phosphate-buffered saline (PBS, pH = 7.4), and Phenylmethanesulfonyl fluoride (PMSF) were purchased from Solarbio Life Sciences (Beijing, China). Penicillin-streptomycin, RPMI 1640 medium, Dulbecco’s modified Eagle medium (DMEM) were purchased from Gibico (Gaithersburg, USA). Certified Fetal Bovine Serum (FBS) was purchased from VivaCell (Shanghai, China). LabPAGE 4–20% was purchased from Beijing LABLEAD BIOTECH Co., Ltd. (Beijing, China). The terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) cell apoptosis detection kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China). The polyfluorene derivative dye P-F8-DPSB (Ex: 405 nm, Em: 525 nm) was gifted from Prof. Fei Huang in South China University of Technology^{38 (link)}. HSPs inhibitors (Tanespimycin and KNK437) were purchased from MedChemExpress.