Facs aria 2

Manufactured by Miltenyi Biotec

The FACSAria II is a high-performance cell sorter designed for advanced flow cytometry applications. It features a sensitive optical design, a robust fluidics system, and a user-friendly interface to enable efficient sorting of complex samples. The core function of the FACSAria II is to rapidly and accurately sort cells based on their physical and fluorescent properties.

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Lab products found in correlation

Magnetic column, by Miltenyi Biotec (1 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Cd8α microbeads, by Miltenyi Biotec (1 mentions) Rna sequencing, by OE Biotech (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Lineage cell depletion kit, by Miltenyi Biotec (1 mentions) Ultra 2 directional rna library prep kit, by Illumina (1 mentions) Hiseq next generation sequencing instrument, by Illumina (1 mentions)

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FACS Aria (9 citations)

3 protocols using facs aria 2

Adoptive Transfer of Antigen-Specific T Cells

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Spleens and LNs were pooled from 10 rBCG-vaccinated donor mice at day 15, and APC-Ag85B:I-Ab CD4⁺ T cells were enriched with anti-APC beads over a magnetic column (Miltenyi Biotec) prior to cell sorting using a FACS Aria II. Endogenous or P25 Ag85B-specific T_EM, T_CM, or T_FH at >85% purity were injected intravenously into recipient mice. Naive Tg P25 cells were enriched from the pooled LNs from CD90.1⁺ congenic mice, using a CD4⁺ T-cell isolation kit (Miltenyi Biotec).

Vogelzang A., Perdomo C., Zedler U., Kuhlmann S., Hurwitz R., Gengenbacher M, & Kaufmann S.H. (2014). Central Memory CD4+ T Cells Are Responsible for the Recombinant Bacillus Calmette-Guérin ΔureC::hly Vaccine's Superior Protection Against Tuberculosis. The Journal of Infectious Diseases, 210(12), 1928-1937.

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Enriching CD8α T cells from MWA-treated tumors

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Briefly, 2 × 10⁶ MC38 cells were subcutaneously injected into the symmetrical bilateral back of male C57BL/6 mice. MWA was performed only on the right flank tumors as mentioned above. After 3 days of MWA treatment, target cells were enriched using CD8α microbeads (Miltenyi) and flow-sorted using FACS Aria II. Total RNA was prepared and subjected to RNA sequencing (Shanghai OE Biotech, Shanghai, China).

Xiao W., Huang H., Zheng P., Liu Y., Chen Y., Chen J., Zheng X., Chen L, & Jiang J. (2023). The CXCL10/CXCR3 Pathway Contributes to the Synergy of Thermal Ablation and PD-1 Blockade Therapy against Tumors. Cancers, 15(5), 1427.

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RNA-Seq Analysis of U2af1-Deleted Bone Marrow

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LSK cells from the BM of control and U2af1-deleted BMT mice, and lineage-negative (Lin^-) cells from the BM of control and primary U2af1-deleted mice were first enriched using Lineage cell depletion kit from Miltenyi and then sorted using a FACS Aria II at 7 days after pI-pC induction. Total RNA was extracted from BM LSK and Lin^- cells using RNeasy Mini kit (Qiagen). RNA sequencing was performed using Ultra II Directional RNA Library prep kit for Illumina (from NEB) and Hiseq next-generation sequencing instrument (Illumina). Gene set enrichment analysis was performed as described [19 ]. To identify splicing events, rMATS [20 ] was used. RT-PCR followed by gel electrophoresis was used to identify splicing events. Image J software was used to quantify the band intensity. Sequences of the primers are available in the supplemental Table 3. RNA-seq data from U2af1 cKO mice will be deposited to publicly available database (GEO). RNA-seq data for MP (Lin^-c-kit⁺) cells from U2af1 S34F knock-in mice: GSE112174.

Dutta A., Yang Y., Le B.T., Zhang Y., Abdel-Wahab O., Zang C, & Mohi G. (2021). U2af1 is required for survival and function of hematopoietic stem/progenitor cells. Leukemia, 35(8), 2382-2398.

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