For MSC differentiation, cells were grown in StemPro adipogenic, osteogenic, or chondrogenic culture medium (Thermo Fisher Scientific, Waltham, MA, USA). Cells were grown in the adipogenic medium for 1 week, osteogenic medium for 1 weeks, and chondrogenic medium for 2 weeks. Adipocytes were stained with oil red O (Sigma-Aldrich, St. Louis, MO, USA) for 10 min, osteoblasts were stained with alkaline phosphatase stain kit (Sigma-Aldrich) for 10 min, and chondrocytes were stained with Safranin O (Sigma-Aldrich) for 5 min. The samples were visualized by inverted microscopy (Nikon, Tokyo, Japan).
Stempro adipogenic
Manufactured by Thermo Fisher Scientific
Sourced in United States
The StemPro adipogenic differentiation kit is a cell culture medium designed for the in vitro differentiation of mesenchymal stem cells (MSCs) into adipocytes. The kit provides the necessary growth factors and supplements to support the adipogenic differentiation process.
Automatically generated - may contain errors
2 protocols using stempro adipogenic
1
Multilineage Differentiation Assay for MSCs
2
Multilineage Differentiation Assay of Mesenchymal Stem Cells
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For MSC differentiation, cells were grown in StemPro adipogenic, osteogenic, or chondrogenic culture medium (Thermo-Fisher Scientific). To detect adipocytes, cells were cultivated in adipogenic medium for 21 days and stained with oil red O (Sigma-Aldrich, St. Louis, MO, USA) for 15 min. To detect osteoblasts, cells were grown in osteogenic medium for 21 days and subjected to alkaline phosphatase staining with an alkaline phosphatase kit (Sigma-Aldrich). Chondrocytes were detected by staining cells grown in chondrogenic medium for 14 days with safranin O (Sigma-Aldrich).
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