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Mouse anti h3k9me2 ab1220

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Mouse anti-H3K9me2 (ab1220) is a primary antibody that specifically binds to the dimethylated lysine 9 residue on histone H3 protein. This antibody can be used in various applications to detect the presence and distribution of this epigenetic histone modification.

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Lab products found in correlation

Sybr green pcr kit, by Thermo Fisher Scientific (2 mentions) Magna chip kit, by Merck Group (2 mentions) Mouse monoclonal anti flag m2 f 3165, by Merck Group (2 mentions) Mouse anti g9a clonal, by R&D Systems (2 mentions) Rabbit anti g9a clonal c6h3, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Anti-H3K9me2 (ab1220) H3K9me2 (ab1220) Mouse anti-H3K9me2 Anti-H3K9me2 Ab1220 H3K9me2 Mouse anti

2 protocols using mouse anti h3k9me2 ab1220

1

Chromatin Immunoprecipitation (ChIP) Analysis

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ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
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2

Chromatin Immunoprecipitation (ChIP) Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
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