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Mouse il 33

Manufactured by Thermo Fisher Scientific
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Mouse IL-33 is a lab equipment product used for the detection and quantification of the mouse interleukin-33 (IL-33) protein. IL-33 is a cytokine that plays a role in the regulation of immune responses.

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Lab products found in correlation

Mouse il 12, by Thermo Fisher Scientific (2 mentions) Mouse tnf α, by Thermo Fisher Scientific (2 mentions) Mouse ifn γ, by Thermo Fisher Scientific (2 mentions) Mouse il 6, by Thermo Fisher Scientific (2 mentions) Human tgf β1, by Thermo Fisher Scientific (2 mentions) Rapamycin, by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) Beclin 1, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions)

Spelling variants of this product

IL-33 (117 citations) Recombinant mouse IL-33 (2 citations)

3 protocols using mouse il 33

1

Investigating Autophagy Regulation

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2,4,6-trinitrobenzenesulfonic acid (TNBS), 3-methyladenine (3-MA), starvation medium EBSS (Earle’s balanced salt solution), chloroquine and rapamycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse IL-33 was purchased from Pepro Tech Inc (London, UK). Antibodies against Beclin-1, LC3B I/II, P62 and β-actin were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Wang Z., Shi L., Hua S., Qi C, & Fang M. (2019). IL-33 ameliorates experimental colitis involving regulation of autophagy of macrophages in mice. Cell & Bioscience, 9, 10.
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2

T Cell Activation and Differentiation

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Spleens were harvested from C57BL/6 mice, single-cell suspensions were prepared, and T cells were activated in culture with 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28. Cells were split 1:2 with fresh media containing 7.5 ng/mL recombinant IL-2 daily starting 2 days after initial culture. After 5 days, the activated T cells were plated at 2 × 105 cells/well in 200 μL media containing mouse IL-7 at 10 ng/mL in 96-well U-bottomed plates. Where indicated, cultures were supplemented with the following cytokines (all from Peprotech): human TGF-β1 at 10 ng/mL, mouse IL-1 at 100 ng/mL, mouse IL-6 at 100 ng/mL, mouse IL-12 at 10 ng/mL, mouse IL-33 at 100 ng/mL, mouse TNF-α at 125 ng/mL, mouse IFN-γ at 10 ng/mL. Cells were then incubated for 72 hours before staining for flow cytometric analysis.
Bromley S.K., Akbaba H., Mani V., Mora-Buch R., Chasse A.Y., Sama A, & Luster A.D. (2020). CD49a Regulates Cutaneous Resident Memory CD8+ T Cell Persistence and Response. Cell reports, 32(9), 108085.
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3

T Cell Activation and Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were harvested from C57BL/6 mice, single-cell suspensions were prepared, and T cells were activated in culture with 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28. Cells were split 1:2 with fresh media containing 7.5 ng/mL recombinant IL-2 daily starting 2 days after initial culture. After 5 days, the activated T cells were plated at 2 × 105 cells/well in 200 μL media containing mouse IL-7 at 10 ng/mL in 96-well U-bottomed plates. Where indicated, cultures were supplemented with the following cytokines (all from Peprotech): human TGF-β1 at 10 ng/mL, mouse IL-1 at 100 ng/mL, mouse IL-6 at 100 ng/mL, mouse IL-12 at 10 ng/mL, mouse IL-33 at 100 ng/mL, mouse TNF-α at 125 ng/mL, mouse IFN-γ at 10 ng/mL. Cells were then incubated for 72 hours before staining for flow cytometric analysis.
Bromley S.K., Akbaba H., Mani V., Mora-Buch R., Chasse A.Y., Sama A, & Luster A.D. (2020). CD49a Regulates Cutaneous Resident Memory CD8+ T Cell Persistence and Response. Cell reports, 32(9), 108085.
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