The content of Interleukin-4 (IL-4) (E-EL-H0101c, Elabscience) and Interferon γ (IFN-γ) (E-EL-H0108c, Elabscience) was detected by Enzyme linked immunosorbent assay (ELISA). Briefly, the ELISA kit was taken out and then kept for 15 minutes. Then samples and standards were supplemented to corresponding wells and cultured for 3 hours. Later, the liquid in the ELISA plate was discarded, and eluent was added for washing the plate three times. Biotin-labeled working solution was added for incubation for 1 hour. Then the liquid in the plate was discarded, and the eluent was added to wash for three times. The enzyme binding working solution was added and cultured for half an hour, and 100 μl of color developing solution was supplemented for incubation in dark at room temperature for 15 minutes. 100 μl of stop solution was added and mixed. The absorbance value was detected within 30 minutes. A standard curve was drawn, and the sample concentration was calculated.
E el h0108c
Manufactured by Elabscience
Sourced in China
The E-EL-H0108c is a laboratory instrument designed for determining the moisture content of samples. It utilizes the Karl Fischer titration method to measure the water content with high accuracy and precision.
Automatically generated - may contain errors
Lab products found in correlation
E el h6156, by Elabscience (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
E el h0101c, by Elabscience (1 mentions)
E el h6008, by Elabscience (1 mentions)
E el h0109c, by Elabscience (1 mentions)
Interleukin 6 (il 6), by Elabscience (1 mentions)
E el h0103c, by Elabscience (1 mentions)
3 protocols using e el h0108c
1
Quantification of Cytokine Levels by ELISA
2
Quantifying LUAD Cytokine Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After homogenizing the frozen LUAD and paracancerous tissues, the levels of interleukin 8 (IL-8), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and IL-6 were tested by ELISA (Elabscience, Wuhan, China: E-EL-H0109c, E-EL-H0108c, E-EL-H6156, E-EL-H6008). According to the guidebook, the cells were found in a microplate reader (Thermo Scientific, USA) at a 450 nm wavelength of absorbance, and the curve was drawn to calculate the relative concentration of the above-mentioned markers.
3
Cytokine Quantification in Cell Culture Supernatant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were plated in a 6-well plate and processed according to groupings. After 48 hours, the cell culture supernatant was collected, centrifuged at 10,000 g at 4 ℃ for 5 minutes, then the supernatant was obtained. Detection was performed according to the operation of the ELISA detection kit (IL-6, E-EL-H6156, IL-10, E-EL-H0103c, IFN-γ, E-EL-H0108c, Elabscience Biotechnology Co., Ltd.)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!