The following monoclonal antibodies and reagents were used with the indicated specificity and appropriate isotype controls: FITC-conjugated goat anti-human F(ab’)2 antibody (Jackson, Pennsylvania), PE-conjugated mouse anti-human CD279 (PD-1) antibody (BioLegend, Santiago, CA), FITC mouse anti-human TCRαβ (BD Biosciences, San Jose, CA), FITC mouse anti-human CD25 (BD Biosciences), PE mouse anti-human CD69 (BD Biosciences), FITC mouse anti-human CD45RA (BD Biosciences), and PE mouse anti-human CD62L (BD Biosciences). All cells were washed, resuspended in 100 μL PBS containing specific antibodies, and incubated for 30 min at 4 °C. Data were acquired on a Beckman Coulter Gallios flow cytometer and analysed using FlowJo version 10 (Tree Star, Ashland, OR).
Fitc mouse anti human tcrαβ
Manufactured by BD
Sourced in United States
The FITC mouse anti-human TCRαβ is a monoclonal antibody that binds to the alpha and beta chains of the T cell receptor (TCR) complex on human T cells. The antibody is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate), allowing for the detection and identification of TCRαβ-positive cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Fitc mouse anti human cd25, by BD (1 mentions)
Pe mouse anti human cd69, by BD (1 mentions)
Fitc conjugated goat anti human f ab 2 antibody, by Jackson ImmunoResearch (1 mentions)
Pe mouse anti human cd62l, by BD (1 mentions)
Gallios flow cytometer, by Tree Star (1 mentions)
Cellquest pro, by BD (1 mentions)
Fitc mouse anti human cd3, by BD (1 mentions)
Attune nxt acoustic focusing cytometer, by BD (1 mentions)
2 protocols using fitc mouse anti human tcrαβ
1
Profiling Immune Cell Phenotypes
2
Expansion and Characterization of Vγ9Vδ2 T-cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Expanded Vγ9Vδ2 T-cells were purified by positive selection using a human anti-TCR γ/δ T-cell MicroBeads kit (MiltenyiBiotec, Auburn, CA, USA), according to the manufacturer’s instructions. The following monoclonal antibodies (mAbs) were obtained from BD Bioscience (San Jose, CA, USA): FITC-mouse anti-human CD3, PE-mouse anti-human TCRγδ, FITC-mouse anti-human TCRαβ, PE-mouse anti-human Vγ9-TCR, FITC-mouse anti-human Vδ2-TCR, PE-Cy7-mouse anti-human CD314 (NKG2D), PE-mouse anti-human ULBP3, PE-mouse anti-human ULBP2/5/6, APC-mouse anti-human MICA/B, and their IgG controls. After expansion, Vγ9Vδ2 T-cells and tumor cell lines were confirmed by flow cytometry using a FACSCalibur and/or an Attune® NxT Acoustic Focusing cytometer and analyzed using CellQuest Pro (BD Biosciences, San Jose, CA, USA) software version 2.7.873.0.
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