Phospho s6k1 thr308

Manufactured by Cell Signaling Technology

Sourced in United Kingdom

The Phospho-S6K1 Thr308 is a primary antibody that specifically recognizes the phosphorylated form of the S6 Kinase 1 (S6K1) protein at the threonine 308 residue. S6K1 is an important downstream effector of the PI3K/Akt/mTOR signaling pathway and plays a crucial role in the regulation of cell growth, proliferation, and metabolism.

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Lab products found in correlation

Supersignal west pico chemiluminescent substrate kit, by Thermo Fisher Scientific (2 mentions) α tubulin, by Merck Group (2 mentions) Pngase f, by New England Biolabs (2 mentions)

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Phospho-S6K1 (13 citations)

2 protocols using phospho s6k1 thr308

Immunoblotting of signaling pathways

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Protein lysates were routinely loaded on 10% polyacrylamide gels. For immunoblotting, the following primary antibodies were used at recommended dilutions: phospho-S6K1 Thr308 (Cell Signalling Technology, Hitchin, UK (CST; no.9205)), S6K1 (CST; no. 9202), phospho-Ser240/244-ribosomal S6 protein (CST; no. 2215)), S6 ribosomal protein (CST; no. 2217), phospho-Ser65-4E-BP1 rabbit polyclonal (CST; no. 9451), 4E-BP1 (CST; no. 9644), phospho-Ser473-Akt (CST; no. 4060), Akt (CST; no. 9272), phospho-Thr202/Tyr204-p44/42 mitogen-activated protein kinase (Erk1/2; CST; no. 4370), p44/42 mitogen-activated protein kinase (Erk1/2; CST; no. 4695) and α-tubulin (Sigma; no. T6199). They were detected with secondary antibodies (Promega, Southampton, UK; nos. W401B and W402B) and visualised using the SuperSignal West Pico Chemiluminescent Substrate Kit (Thermoscientific, Loughborough, UK). PAT4 was deglycosylated with PNGase F (New England Biolabs, Hitchin, UK; no. P0704) according to the manufacturer's instructions.

Fan S.J., Snell C., Turley H., Li J.L., McCormick R., Perera S.M., Heublein S., Kazi S., Azad A., Wilson C., Harris A.L, & Goberdhan D.C. (2015). PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer. Oncogene, 35(23), 3004-3015.

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Immunoblotting of mTOR Pathway Proteins

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Protein lysates were routinely loaded on 10% polyacrylamide gels. For immunoblotting the following primary antibodies were used at recommended dilutions: phospho-S6K1 Thr308 (Cell Signalling Technology [CST #9205]), S6K1 (CST #9202), phospho-Ser240/244-ribosomal S6 protein (Cell Signalling Technology [CST #2215]), S6 ribosomal protein (CST #2217), phospho-Ser65-4E-BP1 rabbit polyclonal (CST #9451), 4E-BP1 (CST #9644), phospho-Ser473-Akt (CST #4060), Akt (CST #9272), phospho-Thr202/Tyr204-p44/42 MAPK (Erk1/2; CST #4370), p44/42 MAPK (Erk1/2; CST #4695) and α-tubulin (Sigma #T6199). They were detected with secondary antibodies (Promega #W401B, #W402B) and visualised using the SuperSignal^® West Pico Chemiluminescent Substrate kit (Thermoscientific). PAT4 was deglycosylated with PNGase F (New England Biolabs; #P0704) according to manufacturer’s instructions.

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