Mice were injected with 40 μg Tf-biotin conjugates (Sigma-Aldrich, Inc., MO) or PBS buffer and sacrificed 6 hr post-injection. Bladders were collected, fixed in 4 % paraformaldehyde for 24 hr and processed for embedding and sectioning (43 (link)). Tissue sections were analyzed by immunohistochemistry using ABC Elite and NovaRed peroxidase substrate kit (Vector laboratories, CA) to visualize Tf-biotin. Parallel bladder sections were stained with Hematoxylin and Eosin and imaged using a 10x magnification microscope for tissue morphology visualization.
Tf biotin conjugates
Manufactured by Merck Group
Sourced in Macao
Tf-biotin conjugates are compounds consisting of transferrin (Tf) covalently linked to biotin. Transferrin is an iron-binding protein, and biotin is a vitamin essential for various metabolic processes. These conjugates can be used for various biological applications that leverage the specific binding interactions between transferrin and its receptors, as well as the high-affinity binding between biotin and streptavidin or avidin.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tf biotin conjugates
1
Visualizing Tissue Biotin Uptake
2
Visualizing Bladder Tissue Uptake
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were injected with 40 μg Tf-biotin conjugates (Sigma-Aldrich, MO) or PBS buffer and sacrificed 6 hr after injection. Bladders were collected, fixed in 4% paraformaldehyde for 24 hr, and processed for embedding and sectioning (43 (link)). Tissue sections were analyzed by immunohistochemistry using ABC Elite and NovaRed peroxidase substrate kit (Vector laboratories, CA) to visualize Tf-biotin. Parallel bladder sections were stained with hematoxylin and eosin and imaged using a 10x magnification microscope for tissue morphology visualization.
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