H3.3 lys 27 to methionine

A 13-year-old boy’s high-grade glioblastoma PBT24 cell line cells were donated by Prof. M. M. Alonso (University of Navarra, Spain) [43 (link)] for the study. A 3-year-old girl’s diffuse intrinsic pontine glioblastoma (DIPG) SF8628 cell line cells—harboring the histone H3.3 Lys 27-to-methionine (Sigma Aldrich, St. Louis, MO, USA)—were also studied [44 ,45 (link)]. The PBT24 cells were cultivated in Roswell Park Memorial Institute 1640 (RPMI), medium (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, St. Louis, MO, USA) containing 100 IU/mL of penicillin and 100 µg/mL of streptomycin (P/S; Sigma Aldrich, St. Louis, MO, USA). The SF8628 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM)–High Glucose Media (Sigma Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, St. Louis, MO, USA) containing 100 IU/mL of penicillin and 100 µg/mL of streptomycin (P/S; Sigma Aldrich, St. Louis, MO, USA) and 2 mM L-Glutamine (Sigma Aldrich, St. Louis, MO, USA). Cells were incubated at 37 °C in a humidified 5% CO₂ atmosphere.

A 13-year-old boy’s high-grade glioblastoma PBT24 cell line cells were donated by Prof. M. M. Alonso (University of Navarra, Spain) [40 (link)] for the study. A 3-year-old girl’s diffuse intrinsic pontine glioblastoma (DIPG) SF8628 cell line cells—harboring the histone H3.3 Lys 27-to-methionine (Sigma Aldrich, St. Louis, MO, USA)—were also studied [62 ,63 (link)]. The PBT24 cells were cultivated in Roswell Park Memorial Institute 1640 (RPMI) medium (Sigma Aldrich, St. Louis, MO, USA). The media were supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, St. Louis, MO, USA) containing 100 IU/mL of penicillin and 100 µg/mL of streptomycin (P/S; Sigma Aldrich, St. Louis, MO, USA). The SF8628 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM)–High-Glucose media (Sigma Aldrich, St. Louis, MO, USA). The media were supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, St. Louis, MO, USA) containing 100 IU/mL of penicillin and 100 µg/mL of streptomycin (P/S; Sigma Aldrich, St. Louis, MO, USA) and 2 mM L-Glutamine (Sigma Aldrich, St. Louis, MO, USA). Cells were incubated at 37 °C in a humidified 5% CO₂ atmosphere.

Pediatric PBT24 cells (donated by Prof. M.M. Alonso; University of Navarra, Spain) [86 (link)] and diffuse intrinsic pontine glioblastoma SF8628 cells harboring the histone H3.3 Lys 27-to-methionine (Sigma Aldrich, St. Louis, MO, USA) [87 ,88 (link)] were studied. The PBT24 cells were cultivated in 1640 RPMI medium (Sigma Aldrich, St. Louis, MO, USA), and the SF8628 cells were grown in DMEM–High-Glucose medium (Sigma Aldrich, St. Louis, MO, USA). The media were supplemented with 2 mM L-Glutamine (Sigma Aldrich, St. Louis, MO, USA), 10% fetal bovine serum (Sigma Aldrich, St. Louis, MO, USA), 100 µg/mL of streptomycin and 100 IU/mL of penicillin (P/S; Sigma Aldrich, St. Louis, MO, USA). Cells were incubated in a humidified 5% CO₂ atmosphere at 37 °C temperature.