Log In Sign up
The largest database of trusted experimental protocols

Ix70 imaging system

Manufactured by Olympus
Visit Supplier

The IX70 imaging system is an Olympus product designed for microscopic imaging and analysis. It features a modular design that allows for customization to meet specific research needs. The core function of the IX70 is to capture high-quality images and data from microscopic samples.

Automatically generated - may contain errors

Lab products found in correlation

Axiovert s100 microscope, by Eppendorf (2 mentions) Spin x filter, by Corning (2 mentions) Femtojet microinjector, by Eppendorf (2 mentions) Femtotip, by Eppendorf (2 mentions)

2 protocols using ix70 imaging system

1

Microinjection and Axonal Transport Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissociated SCG neurons were microinjected using a Zeiss Axiovert S100 microscope with an Eppendorf FemtoJet microinjector and Eppendorf TransferMan® micromanipulator. Plasmids were diluted in 0.5× PBS (without CaCl2 and MgCl2) and filtered using a Spin-X filter (Costar). The mix was injected directly into the nuclei of SCG neurons using Eppendorf Femtotips. Approximately 100 neurons were injected per dish. Injected plasmids were allowed to express for 16 h before CCCP treatment. Plasmids were injected at the following concentrations: 30 ng/μl NMNAT2-EGFP, 30 ng/μl pDsRed2-N1. Time-lapse imaging of axonal transport was performed on an Olympus IX70 imaging system with 100×/1.35 Oil objective. During imaging, cell cultures were maintained at 37 °C and 5% CO2 in an environment chamber. Images were captured at 4 frames per sec for 2 min. Three neurites per condition were imaged in every individual experiment.
Loreto A., Hill C.S., Hewitt V.L., Orsomando G., Angeletti C., Gilley J., Lucci C., Sanchez-Martinez A., Whitworth A.J., Conforti L., Dajas-Bailador F, & Colemana M.P. (2019). Mitochondrial impairment activates the Wallerian pathway through depletion of NMNAT2 leading to SARM1-dependent axon degeneration. Neurobiology of disease, 134, 104678.
+ Open protocol
+ Expand
2

Microinjection and Axonal Transport Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissociated SCG neurons were microinjected using a Zeiss Axiovert S100 microscope with an Eppendorf FemtoJet microinjector and Eppendorf TransferMan® micromanipulator. Plasmids were diluted in 0.5× PBS (without CaCl2 and MgCl2) and filtered using a Spin-X filter (Costar). The mix was injected directly into the nuclei of SCG neurons using Eppendorf Femtotips. Approximately 100 neurons were injected per dish. Injected plasmids were allowed to express for 16 h before CCCP treatment. Plasmids were injected at the following concentrations: 30 ng/μl NMNAT2-EGFP, 30 ng/μl pDsRed2-N1. Time-lapse imaging of axonal transport was performed on an Olympus IX70 imaging system with 100×/1.35 Oil objective. During imaging, cell cultures were maintained at 37 °C and 5% CO2 in an environment chamber. Images were captured at 4 frames per sec for 2 min. Three neurites per condition were imaged in every individual experiment.
Loreto A., Hill C.S., Hewitt V.L., Orsomando G., Angeletti C., Gilley J., Lucci C., Sanchez-Martinez A., Whitworth A.J., Conforti L., Dajas-Bailador F, & Colemana M.P. (2019). Mitochondrial impairment activates the Wallerian pathway through depletion of NMNAT2 leading to SARM1-dependent axon degeneration. Neurobiology of disease, 134, 104678.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!