Human tubular epithelial cells hk 2

Chemical reagents were purchased from WAKO chemicals (Osaka, Japan) and Nacalai Tesk (Kyoto, Japan). Rabbit polyclonal anti-collagen I antibody was purchased from Abcam (Cambridge, UK). Rabbit polyclonal anti-TG1 and -TG2 sera were made by Japan Lamb (Hiroshima, Japan)^{22 (link)}. Horseradish peroxidase and Alexa 594-conjugated anti-rabbit IgG were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA) or Cosmo Bio (Tokyo, Japan), and Invitrogen (Carlsbad, CA, USA), respectively. The 5-(biotinamido) pentylamine (BPA), a biotinylated primary amine substrate for the TGs, was obtained from Pierce (IL, USA), while 4′,6-diamidino-2-phenylindole (DAPI) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Human tubular epithelial cells (HK-2) were purchased from ATCC (Manassas, VA, USA).

Pioglitazone (PIO, purity > 99%), cisplatin (Cis, purity > 99%) and Sirtinol (EX527) were purchased from Sigma (USA) and Selleckchem (USA), respectively. Anti‐SIRT1, anti‐p53, anti‐acetyl‐p53, anti‐cytochrome c, anti‐PUMA‐α, anti‐Bcl‐2, anti‐Bax, anti‐Cleaved‐caspase‐3, anti‐Cleaved‐caspase‐9, anti‐β‐actin and IgG secondary antibodies were all purchased from Cell Signaling Technology (USA). SIRT1 and Caspase‐3 activity kits were purchased from eBioscience. Human tubular epithelial cells (HK‐2) were purchased from ATCC.