For double staining with foxp3 and CD3 (for represent Tregs) [16 (link)], the sections were carried out using paraffin-embedded sections (4 μm) of renal tissues, and incubated with Foxp3 and CD3 primary antibodies individually overnight at 4 °C. The next day, the samples were incubated with rabbit anti-mouse IgG secondary antibody for 1 h at room temperature. The samples were then observed with a fluorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with ISCapture software, and images were taken with a CCD camera (Discovery C15, Olympus, Tokyo, Japan). The numbers of Foxp3+ positive (green) and CD3+ positive (red) cells counted under fluorescent microscopy.
Discovery c15
Manufactured by Olympus
Sourced in Japan
The Discovery C15 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a compact, modular design and supports a wide range of HPLC columns and sample injection methods. The system provides reliable and reproducible results for a variety of sample types and separation tasks.
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Lab products found in correlation
2 protocols using discovery c15
1
Quantifying Renal Treg Cells by Immunofluorescence
2
Renal Tissue Immunofluorescence Analysis
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Immuno uorescence stainings of Fractalkine, p-p38, and Foxp3 proteins were performed on 4 µm-thick para nembedded sections of renal tissues after incubation with a primary antibody targeting Fractalkine (DF12376, 1:80; A nity), Foxp3 (GTX107737, 1:100; GENE TEX), or p-p38 (AF4001, 1:80; A nity) overnight at 4 °C. Next, the samples were incubated with Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) (ZF0516, 1:100; ZSGB-BIO) for 1 h at 24 °C. Then, the samples were observed with a uorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with ISC capture software, and the images were collected with a CCD camera (Discovery C15, Olympus, Tokyo, Japan).
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