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Anti c1qa

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Anti-C1QA is a laboratory reagent used for the detection and quantification of the C1QA protein. C1QA is a component of the C1 complex, which is the first component of the classical pathway of the complement system. This product can be used in various immunological and biochemical assays to study the C1 complex and its role in immune system function.

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Lab products found in correlation

Axio scan z1, by Zeiss (1 mentions) Anti mpo, by Abcam (1 mentions) Anti h3cit, by Abcam (1 mentions)

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Rabbit anti-C1QA (2 citations)

2 protocols using anti c1qa

1

Immunohistochemical Analysis of Immune Markers

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IHC staining was performed using the reported protocol.71 (link) Briefly, formaldehyde-fixed paraffin-embedded (FFPE) sections were dewaxed with methanol, subjected to antigen retrieval, blocked for 30 min, incubated with a 1:100 dilution of an anti-ARG1 antibody (Abcam, Cat# ab92274,RRID:AB_10563668) overnight at 4°C (anti-C1QA, Abcam, Cat# ab189922, RRID:AB_2894866, 1:100; anti-MPO, Abcam, Cat# ab9535, RRID:AB_307322, 1:100; anti-H3cit, Abcam, Cat# ab5103, RRID:AB_304752; anti-CCDC25, Santa Cruz, sc-515201,1:50), and then incubated with HRP-conjugated goat anti-mouse/rabbit IgG (ZSGB-BIO, Cat#PV-6002) at room temperature for 1 h. A DAB kit (ZSGB-BIO, Cat#ZLI-9019) was used for detection. The whole slide was scanned with an automatic digital slide scanning system (ZEISS, Axio Scan.Z1). The intensity of MPO and H3cit expression was measured with Image-Pro Plus software (RRID: SCR_007369).
Hu Z., Hua X., Mo X., Chang Y., Chen X., Xu Z., Tao M., Hu G, & Song J. (2023). Inhibition of NETosis via PAD4 alleviated inflammation in giant cell myocarditis. iScience, 26(7), 107162.
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2

In vitro Macrophage Efferocytosis Assay

Cited 1 time
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To examine Mϕ efferocytosis in vitro, we adapted an established protocol17 (link)–19 (link). Briefly, neonatal cardiomyocytes (or thioglycollate-elicited peritoneal neutrophils) were labeled with DiI (Invitrogen), UV-irradiated (254nm, 7 minutes), and then incubated at 37°C for 2 hours. The resulting apoptotic cells were collected and added to Mϕ cultures. At the appropriate time point (20 or 60 minutes), cells were washed and then collected for flow cytometry or immunocytochemical analyses. For the inhibitor study, anti-IgG or anti-C1qa (Abcam) were added in conjunction with apoptotic cells.
de Couto G., Jaghatspanyan E., DeBerge M., Liu W., Luther K., Wang Y., Tang J., Thorp E.B, & Marbán E. (2019). Mechanism of enhanced MerTK-dependent macrophage efferocytosis by extracellular vesicles. Arteriosclerosis, thrombosis, and vascular biology, 39(10), 2082-2096.
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