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N hexanoyl dl homoserine lactone ahl

Manufactured by Merck Group
Sourced in United States

N-hexanoyl-DL-homoserine lactone (AHL) is a chemical compound used in research applications. It functions as a quorum sensing molecule, which is a type of intercellular signaling mechanism in bacteria. AHL plays a role in the regulation of gene expression in response to changes in cell population density.

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3 protocols using n hexanoyl dl homoserine lactone ahl

1

Microbial Atenolol Interaction Assay

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All the chemicals used in this study were of pharmaceutical grade. The microbiological media were ordered from Oxoid (Hampshire, UK). Atenolol (CAS number: 29122-68-7), N-hexanoyl-DL-homoserine lactone (AHL) (CAS Number: 106983-28-2), thiamine (CAS number: 67-03-8), DL-norepinephrine hydrochloride (CAS Number: 55-27-6), and Dulbecco’s Modified Eagle’s Medium (DMEM) medium were obtained from Sigma-Aldrich (St. Louis, MO, USA). It is worthy to mention that used Atenolol was in form of hydrochloride salt and it was dissolved in water and kept in stocks prior to use in all the next experiments.
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2

Investigating Bacterial Response to Signaling Molecules

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All the used chemicals were of pharmaceutical grade. The microbiological media were purchased from Oxoid (Hampshire, UK). N-hexanoyl-DL-homoserine lactone (AHL) (CAS Number: 106983-28-2), prazosin (CAS number: 19237-84-4), DL-norepinephrine hydrochloride (CAS Number: 55-27-6), thiamine (CAS number: 67-03-8) and Dulbecco’s Modified Eagle’s Medium (DMEM) medium were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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3

Antimicrobial Activity Evaluation

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All the chemicals used were of pharmaceutical grade. Mueller Hinton (MH) agar and broth, Luria–Bertani (LB) agar and broth, Tryptic Soy Agar (TSA), and Tryptone soy broth (TSB) were purchased from Oxoid (Hampshire, UK). Dulbecco’s Modified Eagle’s Medium (DMEM) medium, DL-norepinephrine hydrochloride (CAS Number: 55-27-6), and N-hexanoyl-DL-homoserine lactone AHL (CAS Number: 106983-28-2) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Salmonella enterica serovar Typhimurium (NCTC 12023) and Chromobacterium violaceum CV026 (ATCC 31532) were used in this work.
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