The plasma samples were diluted four times with Calibrator Diluent and DKK1 amount measured in duplicates using the Human DKK1 Quantikine ELISA Kit (DKK100), according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Bound DKK1 signal was developed with 100μl of tetramethylbenzemidine microwell peroxidase substrate system (KPL, #50-76-00). The reaction was stopped with addition of 100μl 1M H3PO4. The optical density was acquired in a microplate reader set to 450 nm and a correct set to 540 nm. DKK1 levels were calculated from a standard curve generated from DKK1 standard in the same plate. Human plasma MCP-1 levels were similarly measured using Human MCP-1 Quantikine ELISA Kit (DCP00, R&D Systems) per manufacturer’s instruction.
Human mcp 1 quantikine elisa kit
Manufactured by R&D Systems
The Human MCP-1 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human Monocyte Chemoattractant Protein-1 (MCP-1) levels in cell culture supernatants, serum, and plasma.
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Lab products found in correlation
4 protocols using human mcp 1 quantikine elisa kit
1
Quantifying DKK1 and MCP-1 in Plasma
2
Quantification of Urinary MCP-1 by ELISA
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Urine MCP-1 was measured using the Human MCP-1 Quantikine ELISA Kit by R&D Systems. Brie y, monoclonal antibody speci c for human MCP-1 had been pre-coated onto a microplate. Standards, controls and samples were prepared, and 200 uL of each was pipetted into the wells in duplicate. The microplate was covered and left to incubate on a microplate shaker at room temperature for two hours. MCP-1 present in the sample bound the immobilized antibody. After washing away any unbound substances three times, an enzyme-linked polyclonal antibody speci c for human MCP-1 was added to the wells and incubated for one hour. Following three washes a substrate solution was added to the wells and incubated for 30 minutes protected from light. Colour development was stopped, and the intensity of the colour was measured using a spectrophotometer to determine the optical density of each well at 450 nm, with wavelength correction at 540 nm.
3
Quantification of Urinary MCP-1 by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine MCP-1 was measured using the Human MCP-1 Quantikine ELISA Kit by R&D Systems. Briefly, monoclonal antibody specific for human MCP-1 had been pre-coated onto a microplate. Standards, controls and samples were prepared, and 200 uL of each was pipetted into the wells in duplicate. The microplate was covered and left to incubate on a microplate shaker at room temperature for two hours. MCP-1 present in the sample bound the immobilized antibody. After washing away any unbound substances three times, an enzyme-linked polyclonal antibody specific for human MCP-1 was added to the wells and incubated for one hour. Following three washes a substrate solution was added to the wells and incubated for 30 minutes protected from light. Colour development was stopped, and the intensity of the colour was measured using a spectrophotometer to determine the optical density of each well at 450 nm, with wavelength correction at 540 nm.
4
Quantification of Urinary MCP-1 by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine MCP-1 was measured using the Human MCP-1 Quantikine ELISA Kit by R&D Systems. Brie y, monoclonal antibody speci c for human MCP-1 had been pre-coated onto a microplate. Standards, controls and samples were prepared, and 200 uL of each was pipetted into the wells in duplicate. The microplate was covered and left to incubate on a microplate shaker at room temperature for two hours. MCP-1 present in the sample bound the immobilized antibody. After washing away any unbound substances three times, an enzyme-linked polyclonal antibody speci c for human MCP-1 was added to the wells and incubated for one hour. Following three washes a substrate solution was added to the wells and incubated for 30 minutes protected from light. Colour development was stopped, and the intensity of the colour was measured using a spectrophotometer to determine the optical density of each well at 450 nm, with wavelength correction at 540 nm.
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