High molecular weight (HMW) adiponectin levels and total adiponectin levels in mouse serum were determined using ELISA (47-ADPMS-E01, ALPCO; and DY1119, R&D Systems), according to the manufacture’s protocol. For serum insulin measurement, mice were i.p. injected with glucose (1.5 g/kg) after 6 hours of fasting, sera were collected at 0, 15, and 30 min after glucose injection, and serum insulin levels were measured by ELISA (90080; Crystal Chem) according to the manufacture’s protocol. Leptin levels in the mouse serum and 3T3-L1 medium were measured by the mouse leptin ELISA kit (DY498–05, R&D Systems). Resistin levels were measured using the resistin ELISA kit (ELM-Resistin, RayBiotech, Inc.). Serum FGF-21 levels were determined using the FGF-21 ELISA kit (KE10042, Proteintech).
Elm resistin
Manufactured by RayBiotech
The ELM-Resistin is a laboratory equipment used for the detection and quantification of the resistin protein. It is a versatile tool that can be utilized in various research applications, including but not limited to, the study of inflammatory processes, metabolic disorders, and the role of resistin in various disease states.
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2 protocols using elm resistin
1
Adipokine Profiling in Mouse Serum
2
Adipokine Profiling in Mouse Serum
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High molecular weight (HMW) adiponectin levels and total adiponectin levels in mouse serum were determined using ELISA (47-ADPMS-E01, ALPCO; and DY1119, R&D Systems), according to the manufacture’s protocol. For serum insulin measurement, mice were i.p. injected with glucose (1.5 g/kg) after 6 hours of fasting, sera were collected at 0, 15, and 30 min after glucose injection, and serum insulin levels were measured by ELISA (90080; Crystal Chem) according to the manufacture’s protocol. Leptin levels in the mouse serum and 3T3-L1 medium were measured by the mouse leptin ELISA kit (DY498–05, R&D Systems). Resistin levels were measured using the resistin ELISA kit (ELM-Resistin, RayBiotech, Inc.). Serum FGF-21 levels were determined using the FGF-21 ELISA kit (KE10042, Proteintech).
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