Sodium dihydrogenphosphate dehydrate

Manufactured by Fujifilm

Sourced in Japan

Sodium dihydrogenphosphate dehydrate is a chemical compound used in various laboratory and industrial applications. It serves as a buffer solution, maintaining a specific pH level in aqueous environments. The compound is a crystalline, white solid that is soluble in water. Its core function is to regulate the acidity or basicity of solutions, making it a valuable tool for researchers and manufacturers across diverse fields.

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5 protocols using sodium dihydrogenphosphate dehydrate

Quantification of 8-OHdG by HPLC

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We obtained 8-OHdG from Sigma (St. Louis, MO). In addition, we obtained dl-isoproterenol hydrochloride, which was used as an internal standard (IS); sodium dihydrogen phosphate dehydrate; and disodium hydrogen phosphate 12-water from Wako Pure Chemical Industries (Osaka, Japan). We purchased ethylene diamine tetra-acetic acid disodium salt (EDTA-2Na) from Dojindo Laboratories (Kumamoto, Japan). Methanol of chromatographic grade was produced by Kanto Chemical Co., Inc., (Tokyo, Japan). Ultra-pure water was produced using the Milli-Q water purification system (Millipore, Bedford, MA). Other chemicals and solvents were of high-performance liquid chromatography (HPLC) grade or reagent grade quality.

Yama K., Shinbo H., Fujikane Y., Mikami C., Machida M, & Miura J. (2022). The Concentration of 8-Hydroxy-2′-Deoxyguanosine in Plasma During the Menstrual Cycle in Young Japanese Women. Women's Health Reports, 3(1), 267-273.

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Fabrication of Silk Fibroin Biosensor

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High quality SF powder (CAS: 9009-99-8) [29 ] was supplied from Matsuda silk farm [29 ] (Tokyo, Japan) with the registered name “Nanofibroin Powder^®”. Phosphate-buffered saline (PBS) reagents (CAS: 169-27185, 168-27155, 167-26125), sodium dihydrogenphosphate dehydrate (CAS: 13472-35-0), β-D glucose (CAS: 492-61-5), gluconolactone (CAS: 90-80-2), and gluconic acid (CAS: 526-95-4) were purchased from FUJIFILM Wako (Tokyo, Japan). N-2-(aminoethyl)-8-aminooctyltrimethoxysilane (AOTMS) was supplied from Shin-Etsu (Tokyo, Japan) with the article number KBM-6803. GOD powder (CAS: 9001-37-0) was purchased from Oriental East (Tokyo, Japan). A 0.2 mm-thick Ti (99.5%) plate used for the extended gate electrode was purchased from Nilaco (Tokyo, Japan) with the article number TI-453382.

Koike K., Sasaki T., Hiraki K., Ike K., Hirofuji Y, & Yano M. (2020). Characteristics of an Extended Gate Field-Effect Transistor for Glucose Sensing Using an Enzyme-Containing Silk Fibroin Membrane as the Bio-Chemical Component. Biosensors, 10(6), 57.

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Erlotinib Quantification Assay Development

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Erlotinib hydrochloride and O-desmethyl erlotinib were obtained from AdooQ BioScience LLC (Irvine, CA, USA). Ethyl 3,4-bis(2-methoxyethoxy)benzoate (EBMB) was obtained from AK Scientific, Inc (Union City, CA, USA). 2,4,6-Trinitrobenzene sulfonic acid was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Horseradish peroxidase (HRP) and 3,3,5,5-tetramethylbenzidine (TMB) were obtained from Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). Sodium hydroxide, hydrochloric acid, sulfuric acid, sodium dihydrogenphosphate dehydrate, disodium hydrogenphosphate 12-water, sodium azide, tris(hydroxymethyl)aminomethane (Tris), 1-ethyl-3,3-dimethylaminopropyl carbodiimide hydrochloride (EDC), N-hydroxysuccinimide, dioxane, ethyl acetate, N,N-dimethylformamide and bovine serum albumin (BSA) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Hydrogen peroxide (30% in water) was obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Yamamoto Y., Saita T., Yamamoto Y, & Shin M. (2018). Quantitative determination of erlotinib in human serum using competitive enzyme-linked immunosorbent assay. Journal of Pharmaceutical Analysis, 8(2), 119-123.

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Enzyme Inhibition Assay Protocol

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6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 1,1-diphenyl-2-picrylhydrazyl (DPPH), dimethylsulfoxide (DMSO), disodium hydrogenphosphate 12-water, sodium dihydrogenphosphate dehydrate, Triton® X-100, and Valinomycin were purchased from Wako Pure Chemical Industries, Ltd., Osaka, Japan. 4-Methylumbelliferyl oleate (4-MUFO) was purchased from Toronto Research Chemicals, Toronto, Ontario, Canada. Acarbose, p-nitrophenyl glucopyranoside (PNGP) and di-potassium hydrogenphosphate were purchased from Nacalai Tesque, Inc., Kyoto, Japan. α-Glucosidase (from Saccharomyces cerevisiae), porcine pancreatic lipase (PPL, type II), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Cetilistat was purchased from Combi-Blocks, Inc., Japan. Potassium dihydrogen phosphate was purchased from Kanto Chemical Co., Inc. Tokyo, Japan, and 2-morpholinoethanesulfonic acid, monohydrate (MES) was purchased from Dojindo Chemical Research, Kumamoto, Japan. Paraformaldehyde was purchased from Chiyoda Inc. and HCS DNA Damage kit was purchased from Thermo Fisher Scientific Inc.
Absorbance was recorded on the Infinite 200 PRO® (Tecan Austria GmBH, Grodig, Austria) spectrophotometer (for DPPH and α-Glucosidase inhibitory assays) and Wallac ARVO™ MX 1420 multilabel counter spectrophotometer (Perkin Elmer Japan, Kanagawa, Japan) (for lipase inhibitory assay).

Elbashir S.M., Devkota H.P., Wada M., Kishimoto N., Moriuchi M., Shuto T., Misumi S., Kai H, & Watanabe T. (2018). Free radical scavenging, α-glucosidase inhibitory and lipase inhibitory activities of eighteen Sudanese medicinal plants. BMC Complementary and Alternative Medicine, 18, 282.

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Antioxidant Compound Protocol Analysis

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FMT, RF, dimethyl sulfoxide (DMSO), imidazole, nitroblue tetrazolium (NBT), p-nitrosodimethylaniline (RNO), Tween 20, disodium hydrogen phosphate 12H 2 O, sodium dihydrogen phosphate dehydrate, dibutylhydroxytoluene (BHT), ammonium acetate, L-tyrosine (Tyr), sodium sulfite (Na 2 SO 3 ), and D-mannitol were purchased from Wako Pure Chemical Industries (Osaka, Japan). Sodium azide (NaN 3 ), L-tryptophan (Trp), L-cysteine (Cys), and L-histidine (His) were purchased from Sigma-Aldrich Japan (Tokyo, Japan). L-Ascorbic acid (vitamin C; VC) was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Methanol (MeOH) and acetonitrile (liquid chromatography grade) were purchased from Kanto Chemical (Tokyo, Japan).

Uchida A., Onoue S., Ohtake H., Seto Y., Teramatsu T., Terajima T, & Oguchi T. (2016). Photochemical Mechanism of Riboflavin-Induced Degradation of Famotidine and a Suggested Pharmaceutical Strategy for Improving Photostability. Journal of pharmaceutical sciences, 105(2).

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