Calcein am pi live dead kit

Hyaluronic acid sodium salt (HA) from Streptococcus equi, horse radish peroxidase (HRP), tyramine hydrochloride, 2-(N-morpholino) ethanesulfonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), N-hydroxysuccinimide (NHS), galactose oxidase (GalOx), and d-galactose were all purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). The dialysis membrane (molecular cutoff ​= ​3500 ​Da) was purchased from Solarbio Technology Co., Ltd. (Beijing, China). Murine bone marrow mesenchymal stem cells (BMSC) were purchased from Cell Bank of Chinese Academy of Sciences, Shanghai, China. Nerve growth factors (NGF) were from Staidson Biopharmaceuticals Co., Ltd. (Beijing, China). Calcein-AM/PI (Live/Dead kit) was obtained from Sigma Aldrich (St. Louis, MO, USA). CCK-8 kit was obtained from US Everbright, China. Alkaline phosphatase (ALP), glutamic-oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) assay kits were sourced from Solarbio Science &Technology Co., Ltd. (Beijing, China). All of the antibodies used in this study were purchased from Proteintech Group Inc. (Wuhan, China).

Hyaluronic acid sodium salt (HA) from Streptococcus equi, peroxidase from horseradish (HRP, ≥160 units per mg, solid), tyramine hydrochloride, 2-(N-morpholino)ethanesulfonic acid (MES), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), galactose oxidase (GalOX, ≥30 units per mg, solid), and d-galactose were all purchased from Aladdin (Shanghai, China). Calcein-AM/PI (Live/Dead kit) was obtained from Sigma Aldrich (St. Louis, MO, USA). The dialysis membrane (molecular cutoff = 3500 Da) was purchased from Spectrum Laboratories (Rancho Dominguez, CA). Mice bone mesenchymal stem cells (BMSC) were obtained from the Cell Bank, Type Culture Collection, Chinese Academy of Sciences, Shanghai, China. CCK-8 was obtained from US Everbright, China.