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Edta coated vacutainer tubes

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EDTA-coated vacutainer tubes are blood collection tubes used to collect and store blood samples. The tubes are coated with EDTA (Ethylenediaminetetraacetic acid), an anticoagulant that helps prevent the blood from clotting during collection and storage.

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Lab products found in correlation

Easysep direct human neutrophil isolation kit, by STEMCELL (2 mentions) Dxh 800, by Beckman Coulter (1 mentions)

Close spelling variants of this product

EDTA tubes (36 citations) Vacutainer tubes (18 citations) EDTA vacutainer tubes (7 citations) EDTA-coated tubes (7 citations) Vacutainer (6 citations) EDTA-coated (2 citations)

2 protocols using edta coated vacutainer tubes

1

Neutrophil Isolation from Human Blood

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Human peripheral blood samples (2–5 mL) in EDTA‐coated vacutainer tubes (Greiner Bio‐One) were obtained from EWS patients and healthy volunteers. Neutrophils were isolated with the EasySep Direct Human Neutrophil isolation kit (StemCell Technologies Inc.) by immunomagnetic negative selection according to the manufacturer's instructions. The number of isolated neutrophils was quantified with a Beckman Coulter DxH800 hematology analyzer (Beckman Coulter Inc.), and the final concentration was adjusted to 107/mL in RPMI without phenol red.
Shukrun R., Baron S., Fidel V., Shusterman A., Sher O., Kollender N., Levin D., Peled Y., Gortzak Y., Ben‐Shahar Y., Caspi R., Gordon S., Manisterski M, & Elhasid R. (2023). Suggested role for neutrophil extracellular trap formation in Ewing sarcoma immune microenvironment. Cancer Science, 115(1), 36-47.
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2

Isolation of Human Neutrophils

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Human peripheral blood samples (2–5 mL) in EDTA-coated vacutainer tubes (Greiner Bio-One, Austria) were obtained from all participants at each time point. Fresh blood was processed within 60 min of withdrawal. Neutrophils were isolated using EasySep Direct Human Neutrophil isolation kit (StemCell Technologies Inc., Canada), based on immunomagnetic negative selection, according to the manufacturer’s instructions. The number of isolated neutrophils was quantified using Beckman coulter DxH800 (Beckman Coulter Inc., CA, USA), and the final concentration was adjusted to 107/ml in RPMI.
Heshin-Bekenstein M., Baron S., Schulert G., Shusterman A., Fidel V., Ben-Shahar Y., Shukrun R., Binenbaum Y, & Elhasid R. (2023). Neutrophils extracellular traps formation may serve as a biomarker for disease activity in oligoarticular juvenile idiopathic arthritis: a pilot study. Arthritis Research & Therapy, 25, 135.
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