Jem 2000fx electron microscope

Sections of plant tissues of approximately 1 mm³ were fixed in the Electron Microscopy Service of the Centro de Biología Molecular Severo Ochoa (CBMSO) with the method described in Fuente et al. [6 (link)]. Samples were observed in the same service with a JEM-1010 transmission electron microscope and in the Centro Nacional de Microscopía Electrónica de Madrid with a JEM 2000FX electron microscope (JEOL, Tokyo, Japan) operated at 200 kV, coupled with an energy dispersive X-ray microanalysis instrument LINK ISIS 300 (Oxford Instruments, Oxford, UK).

The size and morphology of the composite nanochains were characterized by field-emission scanning electron microscopy (FE-SEM) and TEM. FE-SEM measurements were carried out using a JEOL JSM 6330F instrument operating at an accelerating voltage of 15 kV. TEM measurements were carried out using a JEOL JEM-2000 FX electron microscope operating at an accelerating voltage of 200 kV. The samples were prepared by placing small drops of solution containing the dispersed nanochains on a silicon wafer (for FE-SEM) or on 300-mesh holey carbon-coated copper grids (for TEM) and allowing the solvent to evaporate.
Analysis by XRD was performed using a Siemens D-5000 with monochromatic Cu Kα radiation (λ = 1.540562 Å), and EDX measurements were collected using an Oxford EDX attached to the TEM microscope to confirm the structure. All extinction spectra were recorded at room temperature on a Cary 50 Scan UV–visible spectrometer over the wavelength range of 250–1100 nm. The magnetic properties of the nanochains were obtained using a SQUID magnetometer with fields up to 5 T. The temperature-dependent magnetization curves varying between 5 and 400 K were measured in an applied magnetic field of 100 Oe.