Transcription factor staining buffer set

Manufactured by BioLegend

Sourced in United States

The Transcription Factor Staining Buffer Set is a laboratory product designed to facilitate the detection and analysis of transcription factors in cells. The set includes buffers for cell fixation, permeabilization, and staining, enabling researchers to effectively prepare samples for flow cytometric analysis of intracellular transcription factor expression.

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Lab products found in correlation

7 aad, by BioLegend (1 mentions) Zombie aqua, by BioLegend (1 mentions) Dxp athena flow cytometer, by Cytek (1 mentions) Anti mouse cd16 cd32 antibody, by BioLegend (1 mentions) Anti cd3 bv421, by BioLegend (1 mentions) Anti tnf α pe cy7, by BioLegend (1 mentions) Zombie aqua fixable viability kit, by BioLegend (1 mentions)

Spelling variants of this product

Staining buffer (54 citations) Foxp3/Transcription Factor Staining Buffer Set (8 citations) Transcription Factor Buffer Set (7 citations)

2 protocols using transcription factor staining buffer set

Multiparametric Flow Cytometry Analysis

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Liver, PPs, and peripheral blood immune cells were isolated by density gradient centrifugation as previously described. The surface markers were performed according to standard procedures. Dead cells identified by using 7-AAD (Biolegend) or Zombie Aqua (BioLegend) were excluded from analyses. Intracellular cytokine staining was conducted following cell fixation and permeabilization with transcription factor staining buffer set (BioLegend) and incubated with antibodies described in Table 3. Flow cytometry data were analyzed using FlowJo software (Becton Dickinson and Company).Table 3

Antibodies Used for Flow Cytometry

Antibodies	Source	Clone	Dilution
CD45 Brilliant Violet 421	BioLegend, San Diego, CA	30-F11	0.25 mg/million cells
CD3 APC/Cy7	BioLegend, San Diego, CA	17A2	0.25 mg/million cells
CD4 FITC	BD Biosciences	GK1.5	0.5 mg/million cells
CD8a PerCp/Cy5.5	BioLegend, San Diego, CA	53-6.7	1 mg/million cells
CD19 FITC	BioLegend, San Diego, CA	1D3/CD19	0.125 mg/million cells
NK-1.1 PE	BioLegend	PK136	0.25 mg/million cells
LPAM-1 APC	BD Biosciences, Heidelberg, Germany	DATK32	0.5 mg/million cells
IFN-γ PE	Proteintech, China	XMG1.2	0.25 mg/million cells
IL-17A PE	eBioscience, San Diego, CA	eBio17B7	0.125 mg/million cells

IFN, Interferon; IL, interleukin.

Fu S., Ni T., Zhang M., Ren D., Feng Y., Yao N., Zhang X., Wang R., Xu W., Yang N., Yang Y., He Y., Zhao Y, & Liu J. (2023). Cholinergic Anti-inflammatory Pathway Attenuates Acute Liver Failure Through Inhibiting MAdCAM1/α4β7-mediated Gut-derived Proinflammatory Lymphocytes Accumulation. Cellular and Molecular Gastroenterology and Hepatology, 17(2), 199-217.

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Zombie Aqua Fixable Viability Assay

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The Zombie Aqua Fixable Viability Kit (Cat:423 102, BioLegend, USA) was used to remove dead cells, and single cells were blocked with anti‐mouse CD16/CD32 antibody (Cat:156 603, BioLegend, USA). Then, APC‐Fire 750 anti‐CD45 (Cat:103 153, BioLegend, USA), BV785 anti‐CD11b (Cat:101 243, BioLegend, USA), BV421 anti‐CD3 (Cat:100 227, BioLegend, USA), BV605 anti‐CD8a (Cat:100 743, BioLegend, USA), Super Bright 436 anti‐CD279 (PD‐1, Cat:62‐9985‐82, Thermo, USA) were used to stain cell members for 30 minutes. A transcription factor Staining Buffer Set (Cat:424 401, BioLegend, USA) was used to fix and permeabilize the cells. Intracellular markers were stained for 50 minutes with PE anti‐GZMB (Cat:372 207, BioLegend, USA), PE‐Dazzle 594 anti‐IFN‐γ (Cat:505 845, BioLegend, USA), PE‐CY7 anti‐TNF‐α (Cat:506 324, BioLegend, USA), and APC anti‐Perforin (Cat:154 303, BioLegend, USA). Cytek DxpAthena Flow cytometer (Cytek Biosciences, USA) was applied to detect stained cells, and FlowJo software (version 10.8.1) was used to analyze the data.

Li H., Chen J., Li Z., Chen M., Ou Z., Mo M., Wang R., Tong S., Liu P., Cai Z., Zhang C., Liu Z., Deng D., Liu J., Cheng C., Hu J, & Zu X. (2023). S100A5 Attenuates Efficiency of Anti‐PD‐L1/PD‐1 Immunotherapy by Inhibiting CD8+ T Cell‐Mediated Anti‐Cancer Immunity in Bladder Carcinoma. Advanced Science, 10(25), 2300110.

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