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Human se selectin cd62e quantikine elisa kit

Manufactured by R&D Systems
Sourced in United States

The Human sE-Selectin/CD62E Quantikine ELISA Kit is a solid-phase enzyme-linked immunosorbent assay designed to measure human sE-Selectin/CD62E levels in cell culture supernates, serum, and plasma.

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2 protocols using human se selectin cd62e quantikine elisa kit

1

Serum Biomarkers in ELISA Analysis

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The serum levels of ICAM-1, VCAM-1, E-selectin, and S100 protein were measured using the enzyme-linked immunosorbent assay (ELISA) in duplicate. The Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit (DCD540; R&D Systems, Minneapolis, MN, USA), Human sVCAM-1/CD106 Quantikine ELISA Kit (DVC00, R&D Systems), Human sE-Selectin/CD62E Quantikine ELISA Kit (DSLE00, R&D Systems), and Human S100 ELISA Kit (MBS2503148; MyBioSource Inc., San Diego, CA, USA) were used, respectively.
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2

Plasma Biomarkers of Endothelial Function

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Plasma levels of von Willebrand factor (vWF) antigen were measured using a commercial method (HemosIL Von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA, USA) with intra- and inter-assay CVs of <2.3%; the lower detection limit was 2.2%.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
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