A total of 333 Neisseria gonorrhoeae isolates obtained from the Department of Diagnostics of Sexually Transmitted Diseases, Department of Dermatology and Venereology of the Medical University of Warsaw were tested. These strains were isolated from clinical specimens in the years 2010–2014.
N. gonorrhoeae strains were cultured on PolyVitex VCAT3 Chocolate Agar (bioMerieux) and incubated in incubator HCP 105 (Memmert) at 37 °C in a humidified environment with 5% CO2 for 24–48 h. N. gonorrhoeae was identified using criteria including positive oxidase, acid production from glucose, and Gram-negative staining tests. Confirmed N. gonorrhoeae strains were plated on Mueller Hinton Chocolate Agar (MHCA) (Becton Dickinson) and cultured as previously described conditions before the antimicrobial susceptibility test. Isolates were stored at -80° using the Microbank™ system (Pro Lab Diagnostics).
N. gonorrhoeae strains were cultured on PolyVitex VCAT3 Chocolate Agar (bioMerieux) and incubated in incubator HCP 105 (Memmert) at 37 °C in a humidified environment with 5% CO2 for 24–48 h. N. gonorrhoeae was identified using criteria including positive oxidase, acid production from glucose, and Gram-negative staining tests. Confirmed N. gonorrhoeae strains were plated on Mueller Hinton Chocolate Agar (MHCA) (Becton Dickinson) and cultured as previously described conditions before the antimicrobial susceptibility test. Isolates were stored at -80° using the Microbank™ system (Pro Lab Diagnostics).